Bax基因cDNA的分子克隆研究  

Molecular cloning of Bax cDNAQ

作　　者：曾桂生[1] 陈亚兵[1] 陈华 俞春东[1] 蔡毓[1] 温龙平[1] 

机构地区：[1]厦门大学肿瘤细胞工程国家专业实验室

出　　处：《生物技术》1996年第6期7-10,共4页Biotechnology

摘　　要：细胞编程死亡（ｐｒｏｇｒａｍｅｄｃｅｌｌｄｅａｔｈ）是细胞生理性死亡的一种主要形式。Ｂａｘ具有抑制细胞编程死亡的作用。本研究采用ＰＣＲ方法，从人肿瘤细胞ＨＬ—６０ｃＤＮＡ文库中扩增出若干个ｂａｘｃＤＮＡ片段，然后将它们分别与ＰＧＥＭ—Ｔ测序质粒载体连接，并转化到大肠杆菌ＪＭ１０９中去。用蓝／白法筛选重组菌落，经酶切分析及ＰＣＲ鉴定，获得了插入片段大小约为０．４ｋｂ及１．１ｋｂ的ＢａｘｃＤＮＡ重组质粒ＰＢａｘｌ和ｐＢａｘ２。这些片段的测序及表达工作正在进行之中。Programmed cell death is a major way of cell physiological death, and overexpressed Bax gene, one of programmed cell death associated genes. accelerates the progress of programmed cell death. In this paper, three kinds of bax cDNA fragments were amplified from HL-60 cell line by PCR, ligated with PGEM-T directly, and transformed into E. Colt JM109. Screening with blue-white method and identifying by restriction endonuclease analysis and PCR, two recombinant plasmids, pBaxl, which contains a bax cDNA fragment about 0. 4kb, and pBax2, which contains a bax cDNA fragment of 1. 1kb, were obtained respectively. Sequencing and expression of bax cDNAs are currently in progress.

关 键 词：克隆 分子克隆 BAX基因 BAX CDNA 

分 类 号：Q785[生物学—分子生物学]