高原冻伤大鼠骨骼肌腺苷酸含量的变化  

CHANGESINADENINENUCLEOTIDESCONTENTOFMUSCLEINRATSWITHMOUNTAINFROSTBITE

作　　者：刘嘉瀛[1] 孙方人[1] 杨增仁[1] 刘友梅[1] 李凤芝[1] 颜培华[1] 邓峰[1] 张是敬[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所

出　　处：《中国应用生理学杂志》1996年第4期289-293,共5页Chinese Journal of Applied Physiology

摘　　要：本文以ＨＰＬＣ测定了冷冻前后平原冻伤（ＦＮ）、急性缺氧冻伤（ＦＡＨ）和缺氧习服缺氧冻伤（ＦＨＡＣ）大鼠冻肢腓肠肌中三种腺苷酸含量的变化。模拟６０００ｍ间断性缺氧习服４周大鼠腓肠肌ＡＴＰ含量明显减少，ＡＤＰ和ＡＭＰ含量明显增高；而急性缺氧４ｈ大鼠与ＦＮ组大鼠腓肠肌三种腺苷酸含量无明显差异。冻后２４ｈ各组大鼠冻肢腓肠肌三种腺苷酸含量均明显减少。冻后１２０ｈ三种腺苷酸含量均有不同程度的增高：ＦＮ组腓肠肌ＡＴＰ、ＡＤＰ含量恢复较快，ＦＨＡＣ组ＡＭＰ含量明显高于ＦＮ和ＦＡＨ组。除冻前ＡＴＰ／ＴＡ、ＡＭＰ／ＴＡ及冻后ＡＭＰ含量的变化与组织损伤程度一致外，其余未见一致性的变化。讨论了冻伤后腺苷酸减少的可能原因。作者认为能量代谢变化在平原冻伤与高原冻伤发病中的作用不尽相同；ＡＭＰ降解过程中生成的氧自由基在高原冻伤组织损伤中的作用需进一步研究证实。Changesinadeninenucleotidescontentofgastrocnemiusbeforeandaftercoldinjuryweredeter minedbyHPLCin3groupsofrats,namely,frostbiteatnormoxia(FN),frostbiteduringacutehy poxiafor4hoursatasimulatedaltitudeof6000m(FAH)andfrostbiteduringhypoxiaafteracclimation(4hrs/dfor4weeks)atasimulatedaltitudeof6000m(FHAC).AscomparedwithFNgroup,FHACgroupshowedbothmarkedlydecreasedlevelofATPandincreasedlevelsofADPandAMPpre frost bite,whileFAHgroupshowednosignificantdiference.Adeninenucleotidecontentinratsofthesethreegroupswerealreducedsignificantlyat24hpost frostbite,andincreasedatdiferentdegreesat120hpost frostbite.At120haftercoldinjury,ATPandADPcontentofFNgroupincreasedmorequicklythanthatofFAHandFHACgroups,AMPcontentofFHACgroupwasmuchhigherthanthatofFNandFAHgroups.Thetisuedamagecausedbyfrostbitedidn′tcoincidewiththechangesinade ninenucleotidescontentoffrostbitenmuscleinratswithmountainfrostbiteexceptforthechangesinATP/TA,AMP/TAbeforecoldinjuryandAMPcontentaftercoldinjury.Thecausesofadecreaseinadeninenucleotidescontentaftercoldinjurywerediscussed.Itissuggestedthattheroleofchangesinenergymetabolismonpathogenesisofsealevelandmountainfrostbiteisnotthesame,theefectofoxygenfreeradicalformedduringAMPdecomposionontissuedamageofmountainfrostbiteneedfur thertobeverified.

关 键 词：冻伤 腺苷酸 骨骼肌 高原 病理 

分 类 号：R645.02[医药卫生—外科学]