腺苷A_1受体激动剂对兔血一氧化氮水平及急性心肌梗塞范围的影响  

THEEFFECTSOFADENOSINEA1RECEPTORAGONISTONNITRICOXIDELEVELANDACUTEMYOCARDIALINFARCTIONSIZEINRABBITS

作　　者：李小鹰[1] 郑华光[1] 刘玲玲[1] 田亚平[1] 王承斌 

机构地区：[1]解放军总医院老年心内科

出　　处：《中国应用生理学杂志》1996年第4期313-316,共4页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金

摘　　要：为了解腺苷Ａ１受体激动剂ＲＰＩＡ对血一氧化氮（ＮＯ）水平及急性心肌梗塞范围的影响，２０只新西兰兔随机分为四组：Ⅰ，前降支缺血６０ｍｉｎ，再灌注９０ｍｉｎ；Ⅱ，缺血前１５ｍｉｎ给予ＲＰＩＡ（０．３ｍｇ／ｋｇ）ｉｖ，余同Ⅰ组；Ⅲ，两次缺血１０ｍｉｎ，间隔１０ｍｉｎ然后缺血６０ｍｉｎ，再灌注９０ｍｉｎ；Ⅳ，缺血前１５ｍｉｎ给予选择性腺苷Ａ１受体拮抗剂ＤＰＣＰＸ（１．０ｍｇ／ｋｇ）ｉｖ余同Ⅲ组。分别测定缺血再灌注期血ＮＯ水平、心率、平均血压及ｄｐ／ｄｔ，实验结束经ＴＴＣ染色测定心肌梗塞范围。结果显示，ＲＰＩＡｉｖ后血ＮＯ水平迅速上升达基础值二倍，并在整个缺血期持续高于对照组，同时伴有心率（２６．３％）和平均血压（１７．３％）的下降，ｄｐ／ｄｔ测值各组无明显差异。心肌梗塞范围，Ⅰ．２３．１±９．４％，Ⅱ．１２．１±２．２％，Ⅲ．１１．４±３．０％，Ⅳ．２１．４±７．８％。结论：缺血预适应（ＩＰＣ）减少急性心肌梗塞范围，腺苷Ａ１受体激动剂可以达到ＩＰＣ效果，而该受体阻滞可以取消ＩＰＣ效果；血ＮＯ水平的升高有可能是ＲＰＩＡ引起ＩＰＣ效应的原因之一。ThisstudywastotesttheefectsofadenosineA1receptoragonist,R PIA,onendogenousnitrieoxide(NO)andacutemyocardialinfarction(AMI)sizeinanesthetizedrabbits.20rabbitsweredivid edradomlyinto4grcups.Myocardialischaemiawasinducedbyligationofleftanteriordescending(LAD)arteryfor60minfolowedby90minofreperfusion(groupI,n=5).R PIA(0.3mg/kg)wasinfusedi.v.15minbeforeLADoclusion(groupⅡn=5).Receivedtwice10minocusionwith10minintervalandfinalyfolowedby60minocclusionand90minreperfusion(groupⅢ,n=5).Ase lectiveadenosineA1reoeptorantagonistDPCPX(1.0mg/kg)wasinfusedi.v.15minbeforethefirstoclusionasthatingroupⅢ(groupⅣn=5).ThebloodlevelsofNO(nitrite),heartrate,meanar terialpresureanddp/dtweredeterminedduringtheexperiments.TheAMIsizeweredeterminedbyTTCstaining.TheresultsshowedthatthelevelofNO5minafterR PIAinfusionrapidlyincreasedbytwotimesofbaselineandcontinuedtobehigherthanthatinthecontrolgroupduringischaemiaandreperfusionacompaniedwithdecreaseofheartrate(26.3%)andbloodpresure(17.3%).TheAMIsizesinthe4groupswere23.1±9.4%,12.1±2.2%,11.4±30%,21.4±7.8%respectively.ItisconcludedthattheAMIsizecouldbereducedbyisohemicprecoditioningortheinfusionofadenosineA1receptoragonist(R PlA),theefectotwichcouldbeabolishedbytheantagonistofadenosionA1receptor(DPCPX).TheincreaseofNOlevelafterR PIAinfusionmaybeoneofthemachanismsforthepreconditioningefectofR PIA.

关 键 词：心肌梗塞 腺苷A1 受体激动剂 一氧化氮 

分 类 号：R542.220.2[医药卫生—心血管疾病]