D-精氨酸对低氧胁迫下黄瓜幼苗根系多胺含量和无氧呼吸代谢的影响  

Effects of D-arginine on polyamine content and anaerobic respiration metabolism of cucumber seedling roots under hypoxia stress

作　　者：李璟[1] 胡晓辉[1] 郭世荣[1] 贾永霞[1] 杜长霞[1] 

机构地区：[1]南京农业大学园艺学院,南京210095

出　　处：《应用生态学报》2007年第2期376-382,共7页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金资助项目(30571263)

摘　　要：采用营养液水培法,研究了根际低氧胁迫下D-精氨酸(D-Arg)对两个抗低氧能力不同的黄瓜品种根系中多胺含量和无氧呼吸代谢的影响.结果表明,低氧处理下,黄瓜幼苗根系中多胺含量显著增加,无氧呼吸代谢能力提高;与抗低氧能力弱的‘中农八号’相比,抗低氧能力强的‘绿霸春四号’根系中乙醇发酵活性较高,乳酸发酵活性较低;低氧胁迫下,D-精氨酸能显著降低黄瓜幼苗根系中腐胺、亚精胺和精胺含量,根系中乙醇脱氢酶(ADH)和乳酸脱氢酶(LDH)活性增加,乙醇和乳酸含量升高,植株生长受到抑制,而外源腐胺能缓解D-精氨酸的这种作用.说明黄瓜幼苗根系中较高的多胺含量可能有利于缓解低氧胁迫对植株造成的伤害.By the method of solution culture, this paper studied the effects of D-arginine on the seedling roots polyamine content and anaerobic respiration metabolism of two cucumber （ Cucumis Sativus L. ） cuhivars Zhongnong No. 8 and LUbachun No. 4 differed in hypoxia tolerance. The resuits showed that under hypoxia stress, the putrescine （ Put）, spermidine （ Spd ） and spermine （Spm） contents in the seedling roots of test cuhivars increased significantly, and anaerobic respiration accelerated. The ethanol fermentation activity was higher in the seedling roots of hypoxia-tolerant cuhivar Ltibachun No. 4 than in those of hypoxia-sensitive cuhivar Zhongnong No. 8, while lactate fermentation activity had an opposite trend. Comparing with treatment hypoxia, hypoxia plus Darginine decreased the Put, Spd and Spm contents in roots significantly, enhanced the activities of alcohol dehydrogenase （ADH） and lactate dehydrogenase （LDH） and the contents of ethanol and lactate, and inhibited plant growth. Exogenous Put application lessened the effects of D-arginine. Higher level of polyamines in roots could have great benefits for cucumber seedlings to improve their resistance to hypoxia stress.

关 键 词：多胺 D-精氨酸 无氧呼吸 低氧胁迫 黄瓜 

分 类 号：S642.2[农业科学—蔬菜学]