福建家鸭品种的分子遗传多样性  

Molecular genetic diversity of Fujian domestic duck breeds

作　　者：李慧芳[1] 李碧春[1] 马月辉[2] 汤青萍[3] 陈宽维[3] 屠云洁[3] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]中国农业科学院畜牧研究所,北京100094 [3]中国农业科学院家禽研究所,江苏扬州225003

出　　处：《应用生态学报》2007年第2期463-466,共4页Chinese Journal of Applied Ecology

基　　金：国家高技术研究发展计划资助项目(2001AA243081)

摘　　要：通过筛选的28个多态性较好的微卫星标记检测了福建省金定鸭、莆田黑鸭、连城白鸭、山麻鸭4个家鸭品种的遗传多样性.利用等位基因频率计算了各群体的遗传参数、群体间的Nei氏标准遗传距离DS和DA遗传距离,并采用邻近法(NJ)和类平均法(UPGMA)进行聚类分析和比较.结果表明,福建省4个家鸭品种全部群体的平均杂合度为0.5353,遗传一致性较好,应加强各保种场(区)多样性的保护;各品种间的遗传距离远近顺序在两种遗传距离DS和DA的结果中完全一致,以DA和DS为基础分别得到的UPGMA和NJ的聚类结果完全相同,表明在应用微卫星标记分析品种的遗传多样性时,使用更多的微卫星位点,才可以获得更准确更具普遍性的结论.4个家鸭品种的聚类与各品种的经济类型、生态地域分布关系密切.By using 28 micro-satellite markers with better polymorphism, this paper studied the genetic diversity of four Fujian provincial domestic duck breeds Jinding, Putian black, Liancheng white, and Shanma. According to the alleles frequencies, the polymorphic information content, average heterozygosity, anaqular genetic distance （ DA ） and Nei＇ s standard genetic distance （ Ds ） for each breed were calculated. Based on DA and Ds, four dendrograms were obtained by neighborjoining （NJ） and UPGMA methods. The results showed that the average heterozygosity of the four duck breeds was 0. 5353, indicating that the protection of the genetic diversity of these breeds should be strengthened. The orders of the two types of genetic distances among the breeds were accordant, and the dendrograms were the same, reflecting that much more micro-satellite loci should be adopted to obtain more universal conclusions when the genetic diversity was analyzed. The phylogenetic relationships among the four duck breeds were in accordance with their economic types and ecological localities.

关 键 词：鸭 微卫星标记 遗传多样性 遗传距离 聚类分析 

分 类 号：S834[农业科学—畜牧学]