用快速琼脂糖层析纯化磷酸甘油酸激酶及磷酸甘油醛脱氢酶  

Purification of Phosphoglycerate Kinase and Glyceraldehyde Phosphate Dehydrogenase by DEAE-sepharose Fast Flow Chromatography

作　　者：谭天伟[1] 沈忠耀[1] 

机构地区：[1]北京清华大学化学工程系,北京100084

出　　处：《生物工程学报》1996年第4期429-433,共5页Chinese Journal of Biotechnology

摘　　要：研究了用快速琼脂糖离子交换层析(DEAE-Fast Flow Sepharose)结合PEG 4000/Reppal PES双水相体系从黄豆中分离纯化磷酸甘油酸激酶(PGK)及磷酸甘油醛脱氢酶(GAPDH)。控制床层高度(10～20cm),径向放大具有压降低的优点,设计多点进料取代传统的中心管进料,解决了径向流场不均匀的问题。GAPDH的总收率及纯化倍数分别为58％和144,PGK的总收率及纯化倍数分别为41％和44。工艺成本为2.92美元/ku GPADH,具有一定的实用价值。The purification of phosphoglycerate kinase (PGK) and glyceraldehyed phosphate dehydrogenase (GAPDH) from soy bean was studied by a DEAE-Sepharose Fast Flow chromatograhy. When scaling up,the diameters of columns increase but the heights of columns were maintained in the range of 10 to 20cm to avoid the problem of high pressure drop. The recovery of GAPDH and PGK was 57% and 41% , respectively. The purification factor of GPADH and PGK was 144 and 44, respectively. The economical e-valuation indicated that the cost of the process was 2. 09 $ /ku GAPDH, which had some commercial values.

关 键 词：磷酸甘油酸激酶 磷酸甘油醛 脱氢酶 层析 提纯 

分 类 号：Q550.3[生物学—生物化学]