An adpA homologue in Streptomyces avermitilis is involved in regulation of morphogenesis and melanogenesis  

作　　者：ZHAO JinLei WEN Ying CHEN Zhi SONG Yuan LI JiLun 

机构地区：[1]Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University and Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture, Beijing 100094, China

出　　处：《Chinese Science Bulletin》2007年第5期623-630,共8页

基　　金：Supported by the National Basic Research Program of China (Grant No. 2003CB114205)

摘　　要：In Streptomyces griseus, AdpA, the key transcriptional activator in the A-factor regulatory cascade, switches on the transcription of multiple genes required for secondary metabolism and morphological differentiation. Streptomyces avermitilis also contains an ortholog of adpA, which is named adpA-a. To clarify the in vivo function of adpA-a, an adpA-a-disrupted strain was constructed by double crossover recombination. No difference in avermectin production was found between the adpA-a-disruptant and the wild-type strain. However, this disruptant neither formed spores nor produced melanin and its phenotype was restored to the original wild-type by a single copy of the adpA-a gene integrated into the chromosome. This report shows that adpA-a is involved in regulation of morphological differentiation and melanin production in S. avermitilis.In Streptomyces griseus, AdpA, the key transcriptional activator in the A-factor regulatory cascade, switches on the transcription of multiple genes required for secondary metabolism and morphological differentiation. Streptomyces avermitilis also contains an ortholog of adpA, which is named adpA-a. To clarify the in vivo function of adpA-a, an adpA-a-disrupted strain was constructed by double crossover recombination. No difference in avermectin production was found between the adpA-a-disruptant and the wild-type strain. However, this disruptant neither formed spores nor produced melanin and its phenotype was restored to the original wild-type by a single copy of the adpA-a gene integrated into the chromosome. This report shows that adpA-a is involved in regulation of morphological differentiation and melanin production in S. avermitilis.

关 键 词：灰色链霉菌 除虫链霉菌 黑素生成 AdpA同族体 转化致活因子 形态发生 次级代谢 

分 类 号：Q939.132[生物学—微生物学]