大鼠磷脂酶C-γ1前体mRNA剪接的初步鉴定  

Exploration of the alternative splicing variants of rat phospholipase C-gamma 1 pre-mRNA

作　　者：刘忠英[1] 罗深秋[2] 赵永忠[2] 

机构地区：[1]南方医科大学基础医学院组胚教研室,广东广州510515 [2]南方医科大学基础医学院细胞生物教研室,广东广州510515

出　　处：《南方医科大学学报》2007年第2期191-194,共4页Journal of Southern Medical University

基　　金：广东省自然科学基金重点项目(05102580)~~

摘　　要：目的探讨在大鼠中是否存在磷脂酶C-γ1(PLC-γ1)选择性剪接变异体的表达及其可能意义。方法针对在人中已发现的选择性剪接位点(即第30内含子的3'末端与第31外显子交界处)自行设计引物,将大鼠RNA逆转录为cDNA后,再用该对引物进行PCR扩增及序列测定。用此方法初步筛查了大鼠包括胚胎时期、出生早期、成年时期6种脏器组织心、肝、肺、肾、脑和眼球样品共21份。结果在大鼠中未发现与人相似的第一种剪接方式PLC-γ1a(NM_002660)。结论(1)由于不同物种之间的差异,在大鼠中可能不存在与在人类中已发现的PLC-γ1前体mRNA相似的选择性剪接方式,在大鼠中PLC-γ1转录后的剪接主要以与人类相似的第二种剪接方式(PLC-γ1b)为主。(2)在大鼠中PLC-γ1的选择性剪接位点可能与人不同而位于其他位点。Abstract： Objective To explore the expression of phospholipase C-gamma 1 （PLC-γ/1） alternative splicing variants in rats. Methods According to the sequence of human PLCGI splicing variant, specific primers for rat PLC-γ1 were designed and synthesized. The rat RNA was reverse transcribed into cDNA, which was amplified using the specific primers, and the PCR products were sequenced and analyzed using BLAST and bioinformatics methods. Totally 21 rat tissue samples were examined, including the heart, liver, lung, kidney, eyeball, and brain obtained in 3 different embryonic stages, 7 different early posmatal stages, and in adulthood. Result The result did not show that rat PLC-γ1 had the same splicing variant （PLC-γ1a, NM_002660） as human does. Conclusions The same splicing variant of PLC-γ1 detectable in human may not exist in rats, and the pre-mRNA may undergo splicing resulting predominantly in PLC-γ1b mRNA. Very likely, the alternative splicing site of rat PLC-γ1 is not identical to that of human.

关 键 词：磷脂酶C-Γ1 选择性剪接 MRNA 大鼠 

分 类 号：Q75[生物学—分子生物学]