I型胶原支架材料与人脂肪干细胞体外生物相容性研究  

Cellular compatibility of type collagen I scaffold and human adipose-derived stem cells

作　　者：张云松[1] 高建华[1] 鲁峰[1] 朱茗[1] 廖云君[1] 

机构地区：[1]南方医科大学南方医院整形外科,广东广州510515

出　　处：《南方医科大学学报》2007年第2期223-225,共3页Journal of Southern Medical University

摘　　要：目的观察人脂肪干细胞与I型胶原支架材料的生物相容性,为脂肪组织工程选择适宜的种子细胞载体。方法将人脂肪干细胞与I型胶原支架体外培养复合,采用倒置相差显微镜和扫描电镜,观察细胞在支架上黏附、生长及增殖情况并计算细胞黏附率,XTT比色法检测细胞增殖率。结果脂肪干细胞能良好的在支架上黏附、生长和增殖。结论I型胶原支架材料具有良好的细胞相容性,可作为脂肪组织工程的种子细胞载体。Objective To study the cellular compatibility of type Ⅰ collagen scaffold and human adipose-derived stem cells （ADSCs）in order to explore appropriate scaffold materials for adipose tissue engineering. Methods The morphology and function of the ADSCS were observed by inverted phase contrast microscope, scanning electron microscope and XTT assay when cocultured type Ⅰ collagen scaffold with ADSCs in vitro. Cells adhesive rates were also calculated. Result ADSCs were able to attach, grow and proliferate well on the scaffolds. Conclusion collagen I scaffold exhibits excellent cellular compatibility and can be used as a vehicle for adipose tissue engineering.

关 键 词：人脂肪干细胞 组织工程 支架 细胞相容性 

分 类 号：R318.08[医药卫生—生物医学工程]