用组合肽库技术分析HLA-B27的抗原提呈模式  

Analysis of Antigen Motifs Presented by HLA-B27 Using Combinatorial Peptide Libraries

作　　者：蒋黎华[1] 徐红岩[2] 张海静[2] 史桂英[3] 石学耕[3] 李伟毅[1] 

机构地区：[1]上海交通大学医学院上海市免疫学研究所,上海200025 [2]中国科学院上海有机化学研究所,上海200032 [3]上海交通大学医学院细胞生物学教研室,上海200025

出　　处：《中国生物工程杂志》2007年第2期43-46,共4页China Biotechnology

基　　金：国家自然科学基金资助项目(39800131)

摘　　要：HLA分子抗原表位提呈模式的分析,在自身免疫病和肿瘤的病因与治疗研究方面有重要意义。采用组合肽库的策略合成19组ORX7型肽亚库,通过与荧光素标记肽的竞争结合试验,分析了与强直性脊柱炎有强相关的HLA-B27分子的抗原提呈模式。结果显示HLA-B27与P1为不同氨基酸残基的19种肽亚库有相近的结合率,提示P1为非锚定残基;中国人群最常见的两种HLA-B27亚型B*2704和B*2705,在提呈肽表位的P1模式方面存在一些小差异,P1为D或E的肽亚库与HLA-B*2704的结合能力要强一些,而P1为K的肽亚库则与HLA-B*2705的结合能力强一些。为HLA-B27与强直性脊柱炎关联机制的研究提供了线索,为开展HLA分子的抗原提呈模式分析打下了基础。Analysis of antigen motifs presented by HLA molecules is important for the study of autoimmune disease and cancer vaccine. Using the method of combinatorial peptide libraries, nineteen ORX7 peptide sublibraries were synthesized. Through a competition binding assay between fluorescein labeled reference peptide and peptide sublibraries, the antigen motifs presented by HLA-B27 were investigated. The results revealed that the binding proportions of FL-peptide after competed with different peptide sublibraries were similar, suggest that P1 was non-anchor residue for HLA-B27 peptide binding. HLA-B ＊ 2704 and -B ＊ 2705 found in Chinese population existed some differences about their binding motifs on P1 position. D and E are easier to binding with HLA-B ＊2704 compared to B ＊ 2705, K is easier to binding with HLA-B ＊ 2705 compared to B ＊ 2704. This investigation provided some clues for exploring the mechanism of association between HLA-B27 and ankylosing spondylitis.

关 键 词：组合肽库 HLA-B27 抗原提呈竞 争结合试验 

分 类 号：Q939.91[生物学—微生物学]