热带不同生境稀有放线菌分离  

Isolate Rare Actinomycetes From Entironment of Torrid Zone

作　　者：雷湘兰[1] 沈振国[1] 洪葵[1] 阮继生[2] 

机构地区：[1]中国热带农业科学院热带生物技术研究所/热带作物生物技术国家重点实验室 [2]中国科学院微生物研究所,北京100080

出　　处：《生物技术通报》2006年第C00期455-458,463,共5页Biotechnology Bulletin

基　　金：科技部社会公益项目(2004DIB3J072)

摘　　要：通过物理和化学方法对海南清澜港红树林、海南东寨港红树林、湛江红树林、深圳红树林、吊罗山原始森林、儋州橡胶林和海口火山口等7处热带不同生境土壤样品预处理,共分离到114株放线菌。用显微镜形态观察和菌落形态观察对分离到的放线菌初步归类,从中选取13株菌进行16SrDNA序列分析,并构建系统发育树进行类群。由初步的归类结果对热带不同生境稀有放线菌的类群分布进行分析比较,水生环境和非水生环境以及不同的非水生环境之间,发现稀有放线菌类群分布有着明显差异。In this research, soil from different entironment of torrid zone was dealed with physical and chemic method. These torrid zone include mangroves in Qinglan port, Hainan, Dongzai port, Hainan, Shenzhen and Zhanjiang,virgin forest of Diaoluo mountain, Hainan ,rubber woods of Danzhou,volcano in Hainan. 114 isolates were isolated from these samples of soil. Morphology be done by microscope. 13 isolates were putatively classified to the genus level based on 16S rDNA similarity data. Neighbor-joining tree based on almost-complete 16S rRNA gene sequences showing relationships. Result show distribution of rare actinomycetes in different entironment of torrid zone is different.

关 键 词：热带生境 稀有放线菌 分离 分布 

分 类 号：Q93-33[生物学—微生物学]