高碘酸-硝酸银染色法鉴定糖蛋白  

Silver Staining of Glycoproteins on a Polyarcrymide Gel

作　　者：刘翠芳[1] 蒋继志[1] 

机构地区：[1]河北大学生命科学学院,保定071002

出　　处：《生物技术通报》2006年第C00期534-535,共2页Biotechnology Bulletin

基　　金：河北省自然科学基金资助项目(300081);河北省省级重点学科生物工程资助项目

摘　　要：对传统的糖蛋白染色方法（高碘酸-Sehiff法）进行了改进。蛋白质的氧化采用高碘酸法，染色时采用硝酸银染色法。此方法灵敏度是高碘酸-Sehiff法的100倍，而且比高碘酸-Sehiff法节省16～18h。The Staining of Glycoproteins on a Polyarcrymide Gel was developed by silver staining. Before the silver staining, the proteins were oxidized by Periodic acid. The sensitivity of silver staining was as high as 100 folds and the experimental time was shorten by 16 - 18hours in comparison with the Schiff staining.

关 键 词：糖蛋白 聚丙烯酰胺凝胶电泳 银染 染色方法 

分 类 号：Q53[生物学—生物化学]