两种枸杞糖肽促进小鼠脾淋巴细胞增殖作用比较  

A Comparison between the Enhancing Effects of 2 Preparations of Lycium Barbarum Glycopeptide on the Proliferation of Murine Splenic Lymphocytes

作　　者：刘璐[1] 杜光[1] 方建国[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,武汉430030

出　　处：《医药导报》2007年第2期130-132,共3页Herald of Medicine

摘　　要：目的比较两种枸杞糖肽的免疫活性。方法常规制备小鼠脾细胞悬液,分别加入浓度为500,100,10,1μg.mL-1的两种枸杞糖肽样品(实验室提取和工业化生产的枸杞糖肽样品)溶液,空白对照组不加入药物。将各组细胞悬液平均分成两组,其中一组加入10μg.mL-1的ConA(作为阳性对照)。将各细胞悬液置于37℃、5%二氧化碳温箱培养72 h后,分别采用流式细胞仪和MTT比色法对样品进行分析。结果流式细胞仪分析结果显示,两种枸杞糖肽样品均能促进小鼠脾细胞增殖反应,与ConA合用时,均具有协同作用。MTT比色法结果表明:实验室提取的枸杞糖肽样品能明显促进小鼠脾细胞增殖反应,与空白对照组比较,差异有极显著性(P<0.01);工业化生产的枸杞糖肽样品在较大剂量时对脾细胞有明显促增殖作用;与ConA合用时,实验室生产的样品在较大剂量时具有协同作用,工业化生产的样品有明显的协同作用(P<0.05)。结论两种样品均具有较强的免疫活性。Objective To compare the effects of 2 preparations of Lycium Barbarum glycopeptide(LBG) on the proliferation of murine splenic lymphocytes. Methods Suspensions of splenic lymphocytes harvested from BALB/C mice were prepared with the conventional methods.The suspensions were then separately added to the 2 samples of LBG in concentrations of 500,100,10 and 1 μg·mL-1,respectively.(One of the LBG was prepared in the laboratory,the other was an industrial product).The blank controls were added to mediums without reagents.Each of all the cell suspensions was equally divided into 2 groups,to one of which was added 10 μg·mL-1 of ConA,serving as positive control.The cells were then cultured for 72 h at 37 ℃ in an incubator containing 5%CO2.Flow cytometry and MTT colorimetry were separately used to assay the proliferation of the cells. Results The results of flow cytometry revealed that both of the 2 preparation of LBG could enhance the proliferation of the murine splemic lymphocyles and both of them showed synergetic effects with ConA.MTT colorimetry demonstrated that LBG prepared in the laboratory could significantly promote the proliferation of murine splenic lymphocytes as compared with the controls(P<0.01).The LBG as an industrial product was shown to enhance the lymphocyte proliferation only in relatively higher concentrations.Only in relatively high concentrations did the LBG prepared in the laboratory show synergic effects with ConA while the industrially produced LBG manifested definite synergic effects with ConA(P<0.05). Conclusion Both of the LBG preparation were shown to be provided with definite immunocompetence.

关 键 词：枸杞糖肽 免疫活性 

分 类 号：R286[医药卫生—中药学]