玫烟色拟青霉对烟粉虱的致病力测定  被引量:18

Bioassay of Paecilomyces fumosoroseus on Bemisia tabaci

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作  者:黄振[1] 任顺祥[1] 黎崇军[1] 

机构地区:[1]华南农业大学资源环境学院生物防治教育部工程研究中心,广东广州510642

出  处:《华南农业大学学报》2007年第1期40-44,共5页Journal of South China Agricultural University

基  金:国家"973"科技项目(2006CB102005);"十五"国家攻关项目(2004BA509130604);广东省自然科学基金(010312);广州市科技计划联合资助项目(02181-1)

摘  要:用1.0×10^7mL^-1的玫烟色拟青霉Paecilomyces fumosoroseus分生孢子悬浮液侵染烟粉虱Bemisiatabaci的1龄、2龄、3龄、4龄若虫和伪蛹,其累计死亡率分别为81.28%、91.40%、39.26%、24.67%和7.91%,差异显著,其中以烟粉虱的2龄若虫对攻烟色拟青霉最敏感,累计死亡率最高.用1.0×10^5、5.0×10^5、1.0×10^6、5.0×10^6、1.0×10^7、5.0×10^7、1.0×10^8mL^-1的攻烟色拟青霉孢子悬浮液分别处理烟粉虱2龄若虫,以5.0×10^7mL^-1的效果最佳,在第10、12、14d时的累计校正死亡率分别高达89.33%、92.58%和92.69%.不同孢子含量的攻烟色拟青霉对烟粉虱的LC50值差异显著,对烟粉虱的LT50值存在一定的差异.在处理后第2~6d番茄、节瓜、黄瓜、茄子上攻烟色拟青霉对烟粉虱的累计校正死亡率差异不显著,在第8~14d4种寄主植物上烟粉虱的累计校正死亡率差异显著.The concentration of Paecilomycesfumosoroseus spores in 1.0 ×10^7 mL^-1 was used to infect the 1^st, 2^nd, 3^rd, 4^th instars of larvae and pupae of whitefly, Bemisia tabaci. The result from experiment showed that there was significant difference among the accumulate mortality of 81.28%, 91.40%, 39.26%, 24. 67% and 7.91% to the larvae and pupae, respectively. The second instar of whitefly was the most sensitive stage to P. fumosoroseus. The P. fumosoroseus spores with the concentration of 1.0 ×10^5, 5.0× 10^5, 1.0 ×10^6, 5. 0 × 10^6, 1.0 ×10^7, 5.0× 10^7 and 1.0 ×10^8 mL^-1 were used to infect the second instar of larvae. The result indicated that the concentration of 5.0 × 10^7 mL^-1 had the best efficience to the 2nd instar larvae, and the accumulative corrected mortality of B. tabaci was high up to 89.33%, 92. 58% and 92. 69% in 10, 12 and 14 d after treatment, respectively. The result showed there was no significant difference among the accumulative corrected mortality of B. tabaci on tomato, melon, cucumber and eggplant from 2 to 6 d, however, some difference were observed on these four host plants from 8 to 14 d after treatment.

关 键 词:攻烟色拟青霉 烟粉虱 致病力 

分 类 号:S432.4[农业科学—植物病理学]

 

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