羟基磷灰石/丝蛋白复合骨材料的生物相容性  

Biocompatibility of Hydroxyapatite-Silk Fibroin Composite Material for Bone Repair

作　　者：王莉[1] 张亚峰[2] 李春忠[1] 

机构地区：[1]华东理工大学材料科学与工程学院,超细材料制备与应用教育部重点实验室,上海200237 [2]苏州大学附属第一医院骨科,苏州215006

出　　处：《华东理工大学学报（自然科学版）》2007年第1期47-51,共5页Journal of East China University of Science and Technology

基　　金：国家自然科学基金重点项目(20236020);国家基础研究重大项目前期研究专项(2002CCA02200);上海市基础研究重大项目(04DZ14002);上海市重点实验室专项基金(04DZ05622);上海市纳米科技基金项目(0452nm001;0352nm020);教育部留学归国人员科研启动基金(2005383);第三十八批中国博士后科学基金

摘　　要：以共沉淀法制备了羟基磷灰石／丝蛋白（HA／SF）复合骨修复材料，并对材料进行了XRD、TEM、SEM、孔径分布和孔隙率等相关的测试和表征。结果表明：该复合材料的无机组分为20～30nm长、5nm宽的棒状羟基磷灰石（HA）结晶，这些晶粒沿c轴自组装团聚成簇分散在丝蛋白基质中形成三维多孔结构，其孔径分布在0．3～115μm之间，开口孔隙率达66％。该材料植入动物体内，未出现明显的排异反应，在植入部位有连续的骨性连接和新生骨形成，说明HA／SF复合材料具有良好的生物相容性和骨诱导活性。Hydroxyapatite-silk fibroin （HA-SF） composite for bone repair and replacement was prepared by a co-precipitation method. HA-SF composite was examined by XRD, TEM, SEM and mercury porosimetry. The mineral phase in the composite is identified as monophase crystalline HA with low crystallinity. The primary HA crystallites are rod like in shape with a typical size of 20-30 nm in length and 5 nm in diameter. The HA clusters consisting of self assembled crystallites along c axis are dispersed in SF matrix. Consequently, the composite shows a three-dimensional porous network with open porosity of 66% and pores falling in the range of 0.3-115μm in diameter. HA-SF composite does not cause significant inflammation and foreign body reactions after implantation into lumbar cavity of adult goat. The continuous bony joint and new bone formation are observed at the implantation sites. The good biocompatibility and osteoconductivity of HA-SF composite as confirmed by the animal tests together with its boneresembling microstructure suggest that it would be an ideal biomedical material for bone repair.

关 键 词：羟基磷灰石 丝蛋白 复合骨材料 生物相容性 

分 类 号：O614.231[理学—无机化学] R318.08[理学—化学]