圆二色法研究水解胶原与不浸酸铬鞣剂的作用  

Study on No-pickling Chrome Tanning Agent Reacting with Collagen Hydrolysate by CD Spectrum

作　　者：王应红[1] 陈武勇[1] 陈继平[1] 

机构地区：[1]皮革化学与工程教育部重点实验室(四川大学),四川成都610065

出　　处：《皮革科学与工程》2007年第1期3-5,共3页Leather Science and Engineering

基　　金：国家自然科学基金项目(批准号20476064)

摘　　要：通过圆二色谱法对水解胶原在不同pH值条件下α-螺旋等构象的变化及不浸酸铬鞣剂与水解胶原作用后对胶原构象的影响,研究表明:Fasman概率统计预测出水解胶原的α-螺旋结构的存在,在高pH条件下,α螺旋结构有所降低,反平行β折叠片结构有增加趋势。水解胶原在pH4．6时,与不浸酸铬鞣剂作用,其α螺旋结构数量均减少,无规卷曲增加明显,水解胶原较稳定的氢键结构减少。在高pH条件下,不浸酸铬鞣剂的加入,无规卷曲变化不大,但平行β折叠增加明显。The a helix structure of collagen hydrolysate at different pH and the change of structure of no-pickling chrome agent reacting with collagen hydrolysate were investigated . The results showed the probability statistics of Fasman predictive there was α helix conformation in collagen hydrolysate. The α helix structure was fewer and β-sheet of antiparallel increase at high pH. The α helix structure decreased, random coil raised apparently and collagen hydrolysate of stabilization hydrogen bond abated when collagen hydrolysate reacting with no-pickling chrome tanning solution at pH 4. 6. Random coil of Collagen hydrolysate reacting with no-pickling agent didn＇t change obviously, but β-sheet of parallel increased at high pH.

关 键 词：圆二色谱 不浸酸铬鞣剂 Fasman概率统计 水解胶原 构象 

分 类 号：TS513[轻工技术与工程—皮革化学与工程]