红细胞在外周血单个核细胞活化过程中对细胞因子分泌的影响  

Effect of red blood cells on cytokines during activation of peripheral blood mononuclear cells

作　　者：宿振国[1] 周玉明[1] 高梅兰[1] 鞠胜芝[1] 宋向芹[1] 李凤英[1] 

机构地区：[1]山东省滨州医学院附属医院检验科,256603

出　　处：《国际检验医学杂志》2007年第2期117-118,121,共3页International Journal of Laboratory Medicine

摘　　要：目的 通过检测外周血单个核细胞（PBMC）培养液中细胞因子IFN-γ、TNF—α、IL-1β的浓度，观察和分析红细胞在抗CD3单克隆抗体（CD3McAb）诱导PBMC活化过程中对细胞因子分泌的影响，为临床在分离PBMC进行自体淋巴细胞免疫治疗的应用过程中取舍红细胞数量多少为宜提供实验依据。方法 获取健康成年人PBMC，采用人工方法加入不同数量的红细胞，模拟在制备PBMC过程中残余的红细胞数量，然后根据红细胞数量与PBMC中细胞数量之比的不同组成3个不同的实验组（RBC／PBMc=2：1、10：1、50：13组），同时附设单独PBMC为对照组。利用CD3McAb分别对以上4组细胞进行激活，然后检测各组细胞培养液中细胞因子IFN-γ、TNF-α、IL-1β的含量。结果 随着红细胞加入数量的增加，细胞培养液中各细胞因子的浓度有不同程度的上升，具有统计学意义，并且与加入的红细胞数量呈正相关关系。结论当RBC／PBMC≤50：1时，红细胞在CD3McAb诱导PBMC活化过程中对细胞因子IFN-γ，TNF—α、IL-1β的分泌具有一定的促进作用。Objective By measuring the density of IFN-γ,TNF-α,IL-1β,to observe and analyze the effect of red blood cells on cytokines during CD3McAb induced activation of peripheral blood rnononuclear cells（PBMC）,providing the experimental evidence of remaining RBC in the course of isolating PBMC during autolymphocyte therapy（ALT）. Methods Collecting PBMC of healthy adults, by adding RBC to PBMC,to found the three test groups（ RBC/PBMC=2 ： 1,10 ： 1 and 50 ： 1 group respectively）, at the same time, to found the comparison group; PBMC alone. Using CD3McAb to induce the four groups respectively, in the end, to collect conditioned medium for measuring the density of IFN-γ,TNF-α and IL-1β with ELISA. Results With addition of RBC, there was obvious change between the different groups, and there was statistically significant difference. Conclusion When RBC/ PBMC≤50 ： 1, red blood cells can promote the production of IFN-γ,TNF-α and IL-1β in CD3McAb- induced PBMC.

关 键 词：红细胞  抗体 单克隆 抗原 CD3 单核细胞 细胞因子类 

分 类 号：R392.12[医药卫生—免疫学]