红树内生海洋真菌1924^#和3893^#混合发酵次级代谢产物的研究  

Secondary Metabolites of Two Marine-derived Mangrove Endophytic Fungi （Strains Nos. 1924^# and 3893^# ） by Mixed Fermentation

作　　者：朱峰[1] 林永成[2] 丁健华[1] 王新平[1] 黄丽莎[1] 

机构地区：[1]佛山科学技术学院化学与化工系,广东佛山528000 [2]中山大学化学与化学工程学院,广东广州510275

出　　处：《林产化学与工业》2007年第1期8-10,共3页Chemistry and Industry of Forest Products

基　　金：国家863计划资助项目(2001AA624010);广东省自然科学基金资助项目(04300674);广东省科技计划资助项目(2004B30101017);佛山市科技发展专项资金资助项目(2005081871);广州科仪协作网基金资助项目

摘　　要：把混合发酵技术应用于分离自南海红树植物的2株内生海洋真菌(菌株编号为1924#和3893#)的培养,其发酵液乙酸乙酯粗提物表现出明显的杀棉铃虫(Heliothisarmigera Hühner)和中华鳋(Sinergasilus spp.)的活性。从该粗提物分离到2个纯培养未能得到的次级代谢产物A和B,经波谱分析分别鉴定它们为2-甲基水杨酸(A)和环(苯丙-苯丙)二肽(B)。其中优势代谢产物A是粗提物具有杀虫活性的主要原因。Mixed fermentation technique was applied to culture two marine-derived mangrove endophytic fungi （strains Nos. 1924^# and 3893^#） from the South China Sea in this paper. The crude extracts from the fermentation liquids extracted by ethyl acetate showed significant bioactivities of killing pests Heliothis armigera Hühner and Sinergasilus spp. in the primary bioassay. Two secondary metabolites A and B were isolated from this crude extracts, and elucidated by spectral methods as 6-methylsalicylic acid（A） and cyclo（phe-phe） dipeptide （B） respectively. Compounds A and B were not obtained from these two strains by pure fermentation. Compound A was responsible for the significant biocide activities of the crude extracts.

关 键 词：内生海洋真菌 混合发酵 次级代谢产物 杀虫活性 

分 类 号：TQ91[化学工程] O629.72[理学—有机化学]