维生素E对老年小鼠脾细胞转化反应及心、脑脂褐质含量的影响  

The effect of vitamin E on blastogenic response of splenocytes aud lipefuscin conteut in the heart and brain of aged mice

作　　者：马萱钺[1] 刘亚娟[1] 冯彪[1] 甘振威[1] 

机构地区：[1]白求恩医科大学营养教研室

出　　处：《中华老年医学杂志》1996年第6期355-358,共4页Chinese Journal of Geriatrics

摘　　要：目的探讨维生素Ｅ对老年小鼠细胞免疫功能及心、脑脂质过氧化反应的影响。方法３月龄幼年小鼠和１８月龄老年小鼠各饲以含维生素Ｅ（ＶｉｔＥ）５００×１０－６和３０×１０－６饲料８周后，测定血清ＶｉｔＥ水平、脾细胞转化反应和心、脑脂褐质含量。结果饲以含５００×１０－６ＶｉｔＥ饲料的老年小鼠脾细胞对刀豆蛋白Ａ（ＣｏｎＡ）和脂多糖（ＬＰＳ）的反应及血清ＶｉｔＥ水平均较饲以含３０×１０－６ＶｉｔＥ饲料者显著增高（Ｐ值＜０．０１）；心、脑组织中脂褐质含量则明显降低（分别为Ｐ值＜０．０１及０．０５）。结论膳食中补充较高剂量ＶｉｔＥ后，能显著提高老年小鼠血清ＶｉｔＥ水平，增强脾细胞转化反应，并明显抑制心、脑脂质过氧化。Objective In this study, the effect of vitamin E (Vit E) on cell-mediated immune functions and lipid peroxidation in the brain and heart of aged mice were observed. Methods Semi-purified diet containing 30×10(-6) or 500×10(-6) DL-α-tocopherol (Vit E) was fed for 8 weeks to young (3 months) and old ( 18 months) Swiss mice. The average daily intake of Vit E was 42mg/kg or 2.5mg/kg body weight for the young and 30mg/kg or 1.8mg/kg body weight for the aged. The blastogenic response of splenocytes as well as the serum Vit E and lipofuscin levels in brain and heart were measured. Results It is shown that the old mice fed with 500×10(-6) Vit E diet had a significantly higher serum Vit E level and blastogenic response of splenocytes to Con A and LPS than those fed with 30× 10(-6) Vit E diet (P<0.01). However, lipofuscin level in the brain and heart of the aged mice declined substantially by Vit E supplementation (heart:P<0.01, brain:P< 0.05 ).Conclusions Higher dose of dietary Vit E supplementation significantly enhanced the serum Vit E level and blastogenic response of splenocytes of aged mice,while obviously inhibited lipid peroxidation and thereby reduced lipofuscin accumulation in the tissues.

关 键 词：维生素E 脾细胞 转化率 心 脑 脂褐质 

分 类 号：R977.25[医药卫生—药品]