伤害性刺激电针后中缝背核生长抑素mRNA的表达及其与5-HT的共存  

Expression of SOM mRNA and Coexistence of SOM mRNA and 5-HT in Nucleus Raphe Dorsalis Following Noxious Stimulation and Electroacupuncture Analgesia

作　　者：方智慧[1] 吴爱群[1] 朱君明[1] 楚宪襄[1] 王金兰[1] 范天生[1] 

机构地区：[1]河南医科大学人体解剖学教研室,郑州450052

出　　处：《针刺研究》1996年第3期22-26,共5页Acupuncture Research

摘　　要：本实验用原住杂交和免疫组化ABC相结合的双标记技术，观察了大鼠中缝背核（RD）内生长抑素（SOM）fllRNA在正常、伤害性刺激、单纯电针和电针镇痛（EA）下的变化特点。结果发现RD正常情况下有一定SOMmRNA表达，伤害性刺激和单纯电针后SOMmRNANA表达增强，EA后进一步增强。在正常情况下RD内有少量的SOMmRNA与5－HT双标记细胞。与正常组相比，伤害性刺激和单纯针刺后双标细胞增多，EA后更甚。结果提示：（1）SOM可能参与痛觉调杯（2）SOM可能参与EA。（3）RD内SOMmRNA与SNT共存，二者可能协同作用，共同参与EA。Using the methods of in situ hybridization (ISHH), ISHH combined with immunocytochemistryABC and image processing, somatostatin (SOM) mRNA level and coexistence of SOM mRNA and 5-HT in the nucleus raphe dorsalis (RD) of rat were studied in the normal group, noxious stimulationgroup, simple electroacupuncture group and electroacupuncture analgesia group (EA).A number of oval and fusiform cells with a weak to moderate hybridization signals were mostlydistributed in dorsomedial subnucleus of RD and concentrated on rostral part. An increase of SOMmRNA positive cells could be seen in noxious stimulation group and simple electroacupuncture groupcomparing with the normal group. In EA group the number of SOM mRNA positive cells increasedmore than any other groups.A few SOM mRNA and 5-HT doubled cells could be seen in RD in the normal group. Most of thedoubled cells were distributed in ventromedial, lateral subnuclei of RD. Chmparing with the normalgroup, doubled cells increased in noxious and simple electroacupuncture group and increased signifi-cantly in EA group. The results suggest: (1) SOM takes part in modulation of pain. (2) SOM playsa role in EA. (3) Coexistence of SOM mRNA and 5-HT in RD suggests that SOM and 5-HT interactsynergically in EA.

关 键 词：中缝背核 电针镇痛 生长抑素 

分 类 号：R245.97[医药卫生—针灸推拿学] R246.2[医药卫生—中医临床基础]