大鼠烧伤早期血浆内皮素变化及其对内皮细胞通透性的影响  

Dynamic changes of endothelin in sera of rats at early burn stage and its effects on endothelial cell permeability in

作　　者：高建川[1] 杨宗城[1] 黎鳌[1] 罗勤[1] 罗向东[1] 潞淑珍 郭振荣 

机构地区：[1]第三军医大学西南医院烧伤研究所,重庆630038 [2]北京解放军第三○四医院烧伤中心

出　　处：《中华整形烧伤外科杂志》1996年第6期419-422,共4页

基　　金：国家自然科学基金资助课题

摘　　要：为探讨烧伤早期内皮素变化及不同浓度ET-1对培养血管内皮细胞通透性的影响,动态观察了大鼠30％Ⅲ度烧伤早期血浆内皮素变化规律及其对内皮细胞通透性的直接影响。结果表明:血浆ET浓度伤后迅速升高,6小时达峰值,以后虽有所回落,但24小时内仍显著高于基础水平(P<0.01);培养血管内皮细胞单层与0,1,10,100nmol/L ET-1孵育后,其对Hank’s液或白蛋白灌注液的滤过流量、滤过系数均明显增加,而对蛋白渗透压反射系数显著减少,且存在剂量依赖性。提示烧伤后异常增高的ET除发挥其强大的缩血管作用,调节血流分布外,还直接促进了烧伤后毛细血管通透性的增加。The dynamic changes of endothelin in sera of early burnt rats and the effects of endothe- lin-1 on the permeability of vascular endothelial cells were investigated. In the early phase of 30% ( Ⅲ degree) total body surface area (TBSA) burn rat model, we measured the concentration of endothelins in plasma, the water contents in main organs and the permeability of endothelial monolayer cultured on micropore membrane to Hank's solution or 5 g/L albumin Hank's solution. Endothelin concentration in burnt rat sera was firstly increased dramatically,showing a peak at 6 hours postburn, and then remained significantly higher than the basic level(P<0.01). The changes of endothelin were correlated with the alteration in water contents in main organs after burn. Moreover, if the cultured endothelial cells were exposed to 0,1,10,100 nmol/L endothelin 1 for 60 min, it was found that the filtration volume (Jv) and the filtration coefficient (Kf) of endothelial monolayer were significantly enhanced as determined by ei- ther Hank's or albumin (5g/L) perfusion, while the osmotic reflection coefficient (o) (to protein) de- creased in a dose-dependent pattern. The present results suggest that the postburn elevated endothelin may not only mediate the blood flow distribution via potent vasoconstriction, but also directly increase the vascular permeability. The mechanism(s) of the latter event needs to be further clarified.

关 键 词：内皮素 血管内皮 烧伤 

分 类 号：R644.02[医药卫生—外科学]