五指山猪生长激素基因全序列克隆及序列分析  

Molecular Cloning and Sequence Analysis of Growth Hormone Gene for WZS-Miniature Pig

作　　者：林莉[1] 欧江涛[2] 郭春华[1] 黄礼光[2] 王希龙[2] 

机构地区：[1]西南民族大学生命科学院 [2]海南省农科院畜牧所,海口510031

出　　处：《西南师范大学学报（自然科学版）》2007年第1期78-81,共4页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：西南民族大学重点科研资助项目(03NZ008);海南省自然科学基金(琼科[2003]255).

摘　　要：通过筛选一对引物,PCR扩增五指山小型猪GH基因全序列,并进行克隆测序分析、序列分析及氨基酸分析．结果表明,有98个位点不同,同源性为95．026%,外显子有2个碱基发生了突变,其中外显子2有1个位点的碱基突变导致了氨基酸异义取代．The whole-length sequence of GH gene in WZS-miniature pig was amplified with PCR, using a pair of selected primers, and the amplified DNA fragment was directly sequenced by cloning. Alignment with the sequence reported by Peter Vized in 1987 showed that 98 bp differences were present in the full sequence, the homology between them being 95.26%, and in the 5 exons only 2 bp differences were detected.

关 键 词：五指山猪 生长激素基因 克隆 序列分析 

分 类 号：S858.28[农业科学—临床兽医学]