HAART治疗HIV-1慢性感染儿童外周血特异性CTL的反应特点  

Characters of HIV-1-specific CD8 T-cell responses in peripheral circulation of chronically HIV-1-infected children undergoing highly active antiretroviral therapy

作　　者：金磊[1] 赵清霞[2] 张政[1] 福军亮[1] 何云[2] 姚金霞[1] 王福生[1] 

机构地区：[1]解放军第302医院生物治疗研究中心,北京100039 [2]郑州市第六人民医院感染科

出　　处：《解放军医学杂志》2007年第2期107-109,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：目的观察高效抗逆转录病毒治疗(HAART)前后人类免疫缺陷病毒Ⅰ型(HIV-1)慢性感染儿童外周血HIV-1特异性CTL的反应特点,并分析其影响因素。方法采集24例HIV-1慢性感染儿童外周血并分离PBMCs,常规进行CD4T细胞绝对数和血浆病毒载量测定。应用tetramer染色技术和ELISPOT技术分别测定HIV-1表位特异性CTL频率和IFN-γ产生细胞的数量。结果在80%的HIV-1慢性感染儿童外周血可检测到HIV-1特异性的tetramer+CTL细胞和HIV-1表位肽刺激的IFN-γ产生细胞。未经HAART治疗和HAART治疗失败的儿童,其体内CTL反应明显强于HAART治疗成功的儿童,并且这种CTL反应与HAART治疗时间呈明显的负相关。结论HAART治疗后外围血HIV-1特异性CTL反应明显降低,持续的HIV-1抗原刺激是维持外周血HIV-1特异性CTL反应的主要因素。Objective To characterize the properties of human immunodeficiency virus-1- （HIV-1） specific cytotoxic T lymphocyte （CTL） responses, and to analyze the factors which may affect CTL responses before and after treatment with highly active antiretroviral therapy （HAART） in children with chronic HIV-1 infection. Methods A total of 24 HIV-1-infected children were studied. Circulating CD4 T cell counts and HIV-1 RNA levels were routinely measured. Peripheral HIV-1-specific CTL frequency and the specific interferon-γ- producing lymphocytes were determined by tetramer staining and enzyme-linked immunospot （ELISPOT） assay, respectively. Results It was found that HIV-1-specific CTL responses could be detected in vitro in more than 80% of Chinese children with HIV-1 infection. Circulating CTL, frequency and IFN-γ-producing lymphocyte counts were significantly reduced in HAART suppressed HIV-1 infected children in comparison with HAART failure children and HAART na？ ve children. The duration of virus suppression after HAA-RT was found to be inversely correlated with CTL frequency. Conclusion HIV-1-specific CTL response to virus was warranted by antigenic stimulation. The present work may elucidate the immune mechanisms influencing the outcome of HIV-1 carriers with treatment of HAART.

关 键 词：T淋巴细胞 细胞毒性 树突细胞 HIV 儿童 

分 类 号：R512.91[医药卫生—内科学] R392.11[医药卫生—临床医学]