用于禽流感H9亚型检测的压电免疫蛋白芯片的研制  

A piezoelectric immunity protein chip for determination of H9 subtype avian influenza(AIV) viruses

作　　者：刘毅新[1] 刘仲明[1] 王弘[2] 孙远明[2] 

机构地区：[1]广州军区广州总医院,广州510010 [2]华南农业大学食品学院,广州510642

出　　处：《中国卫生检验杂志》2007年第2期217-218,230,共3页Chinese Journal of Health Laboratory Technology

基　　金：广东省重大科技专项资助(2005A2090101)

摘　　要：目的：研制禽流感H7亚型检测的压电免疫蛋白芯片。方法：采用聚乙烯亚胺（PEI）粘附、戊二醛（Glu）交联的方法，将禽流感H9抗原固定于石英晶体的金电极表面。研究了敏感材料的浓度、pH值对其固定的影响。结果：该芯片用于检测浓度范围为0．123～1．23mg／ml的禽流感H9血清时，呈线性相关，相关系数0．9854。结论：检测阳性样品的响应值与阴性样品的噪声值之比大于2，可用于定性定量检测。Objective ： To research the piezoelectric immunity protein chips for the detection of H9 subtype AIV. Methods： The H9 subtype avian influenza virus antigen was fixed on gold electrode surface of the quartz crystal by polyethylenimine adhesion and glutaraldehyde cross - linking method. The effect of the concentration of sensitive matrial and pH value of immobilization was determined. Results： The resonant range of the sensor was between 0. 123 - 1.23 mg/ml and the correlated coefficient 0. 9854. Conclusion ：The sensitivity for positive serum is 2 times higher than negative serum,which can be used to qualitative and quantitative detection of H9 subtype AIV.

关 键 词：禽流感H9亚型 戊二醛交联法 压电石英晶体 免疫传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]