慢性乙肝患者CD25及CD2m5RNA表达及临床意义  

Expression of CD25,CD25 mRNA in patients with chronic hepatitis B and its clinical significance

作　　者：王瑜[1] 刘新矿[2] 王健[1] 

机构地区：[1]安徽理工大学医学院病原生物学与免疫学教研室,安徽淮南232001 [2]安徽省淮南市第一人民医院感染科,安徽淮南232007

出　　处：《中国卫生检验杂志》2007年第2期302-304,共3页Chinese Journal of Health Laboratory Technology

基　　金：安徽省教育厅自然科学基金项目(2006kj304B)

摘　　要：目的:探讨慢性乙肝患者CD25、CD25 mRNA表达水平及其临床意义。方法:选择249例典型慢性乙肝患者,用生物素-链霉亲和素(BSA)法和Real-tim e PCR法分别检测PHA诱导前后患者PBMC CD25和CD25 mRNA表达水平;用PCR法检测患者HBV-DNA载量。结果:慢性乙肝患者静息态和诱导态CD25、CD25 mRNA表达水平与正常对照组相比均降低,差异有显著性(P<0.05或P<0.01)。慢性乙肝、肝硬化患者CD25和CD25 mRNA的表达水平不完全相同,但差异无显著性(P>0.05)。结论:乙肝患者存在一定程度的免疫功能低下,CD25、CD25 mRNA表达水平降低,其表达水平与病情活跃程度、病毒复制水平相关。Objective：To explore the expression of CD25 and its mRNA in patients with chronic hepatitis B and its clinical significance. Methods：The expressions of CD25 and its mRNA before and after being stimulated with PHA in PBMC of 249 patients with typical chronic hepatitis B were detected by the biotin- streptavidin （BSA） technique, real -time PCR, respectively. The levels of HBV - DNA in serum and PBMC were dynamically detected by PCR. Results：The level of CD25 and its mRNA either in silence or in inducement was lower in patients with chronic hepatitis B than that in normal controls （ P 〈 0. 05 or P 〈 0. 01 ）. However, there was little discrepancy between patients with chronic hepatitis B and those with cirrhosis （P 〉 0. 05 ）. Conclusion：The immune function is low in the patients with chronic hepatitis B, and low expression of CD25 and its mRNA in patients is associated with pathogenetic condition and the replication of HBV - DNA.

关 键 词：HBV—DNA CD25 MRNA Real—time PCR PCR 

分 类 号：R512.62[医药卫生—内科学]