OPA柱前衍生法测定硫酸新霉素的含量  

Determination of neomycin sulfate by OPA pre-column derivatization

作　　者：李锋[1] 邱小明[1] 石贤爱[1] 郭养浩[1] 

机构地区：[1]福州大学生物科学与工程学院,福建福州350002

出　　处：《福州大学学报（自然科学版）》2007年第1期130-132,共3页Journal of Fuzhou University(Natural Science Edition)

基　　金：福建省科技厅资助项目(2004HZ031)

摘　　要：建立了一种硫酸新霉素的HPLC分析方法.以邻苯二甲醛(OPA)为衍生化试剂进行柱前衍生化,334nm作为检测波长,流动相为甲醇水溶液(V甲醇∶V水=70∶30),标准曲线为Y=1×106X+4.123 8×104,r=0.998 3,平均回收率为99.48%.用该方法测定发酵液中硫酸新霉素的含量取得了满意的结果.A HPLC method for the determination of neomycin sulfate was established. In this method, the derivative regents are OPA and thioglycol, 334nm was selected as the detection wavelength, the mobile phase was methanol -water（ Vmathanol ： Vwater = 70： 30）, standard line： Y = 1 × 10^6X ＋ 4. 123 8×10^4, r = 0. 9983, the average recovery is 99.48%. This method can be applied to determinate the neomycin sulfate in fermented broth with satisfactory results.

关 键 词：硫酸新霉素 邻苯二甲醛(OPA) 柱前衍生化 

分 类 号：R927.2[医药卫生—药学]