槲皮素及异槲皮素、芦丁抗自由基活性的比较研究  

Comparative study on in vitro anti-free radical activities of quercetin, isoquercetin, and rutin

作　　者：金越[1] 吕勇[1] 韩国柱[1] 孙慧君[1] 鱼红闪[2] 金凤燮[2] 

机构地区：[1]大连医科大学药理教研室,辽宁大连116027 [2]大连轻工学院食品与生物工程系,辽宁大连116034

出　　处：《中草药》2007年第3期408-412,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30371744;30470055;20476017)

摘　　要：目的评价3种黄酮类同系化合物槲皮素及其单糖苷异槲皮素和双糖苷芦丁的体外抗自由基作用,进一步研究其构效关系。方法采用H2O2/Fe2+体系,通过Fenton反应生成羟自由基(·OH);采用邻苯三酚自氧化法产生超氧阴离子(O2-)。利用·OH造成肝细胞脂质过氧化以及红细胞膜破裂,观察受试化合物的保护作用,并计算IC50。结果在以上4种体外模型中,3种药物均表现出极强的清除自由基能力。在·OH以及O2-清除实验中,抗氧化活性顺序为芦丁>异槲皮素>槲皮素;在抑制肝匀浆脂质过氧化及红细胞膜破裂实验中,抗氧化活性顺序为槲皮素>异槲皮素>芦丁。结论3种受试黄酮类化合物均具有十分强大的自由基清除作用,且呈现明显的剂量依赖性和糖基结构依赖性,在不同的实验体系中其抗自由基活性强弱顺序不同。Objective To assess in vitro anti-free radical activities of three flavonoids： quercetin, its monoglycoside isoquercetin, and diglycoside rutin, and further investigate their structure-effect relationship. Methods Hydroxyl free radical （ · OH） was generated using H2O2/Fe^2＋ system via Fenton reaction, superoxide anion （O2^-） using pyrogallol auto-oxidation method. Hepatocellular lipid peroxidation and the rupture of RBC membrane were caused by · OH. IC50 was calculated and used to examine the anti-free radical activity. Results Among above four different in vitro models, three flavonoids showed extremely potent free radical-scavenging effects. Rutin demonstrated the greatest potency in hydroxyl free radical and superoxide anion （O2^-） models and quereetin demonstrated the lowest while the reversed results was observed in · OH-caused hepatoeellular lipid peroxidation and · OH-caused rupture of RBC membrane models. Conclusion Three flavoniods have potent anti-free radical effects in a dose- and glyeosyl structure-dependent manner. The anti-free radical activity potency may differ depending on employed experiment system.

关 键 词：黄酮化合物 自由基 槲皮素 异槲皮素 芦丁 

分 类 号：R286.75[医药卫生—中药学]