龙葵碱对荷瘤小鼠红细胞免疫功能的影响  

Effect of Solanum nigrum alkaloid on erythrocyte immunity function in tumor-bearing mice

作　　者：季宇彬[1] 万梅绪[1] 高世勇[1] 邹翔[1] 

机构地区：[1]哈尔滨商业大学药物研究所博士后科研工作站,黑龙江哈尔滨150076

出　　处：《中草药》2007年第3期412-414,共3页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30400591);黑龙江省自然科学基金资助项目(D2004-13);哈尔滨市青年科学基金资助项目(2004AFQXJ035);黑龙江省研究生创新科研资金项目(YJSCX2005-134HLJ)

摘　　要：目的探讨龙葵碱对S180及H22荷瘤小鼠红细胞免疫功能的作用。方法观察S180荷瘤小鼠和H22荷瘤小鼠的红细胞免疫黏附肿瘤细胞能力,采用DPH荧光探针,用荧光偏振法测定荧光偏振度(P),并计算膜的微黏度(η)研究红细胞膜脂流动性(LFU)。结果龙葵碱能升高S180和H22荷瘤小鼠的红细胞黏附肿瘤细胞的花环率,提高两种荷瘤小鼠的红细胞膜的流动性。结论龙葵碱可能是通过提高两种荷瘤小鼠的红细胞膜的流动性,从而恢复荷瘤小鼠红细胞免疫功能进而达到抗肿瘤作用。Objective To study the effect of Solanum nigrurn alkaloid on erythrocyte immunity function. Methods Studying the adherence between erythrocyte immunity and tumor-cell of S180 and H22 tumor-bearing mice; By determining with fluorescence polarization （P） and accounting the micro viscosity （7） with DPH as a probe the erythrocyte membrane fluidity of the S180 and H22 tumor-bearing mice could be investigated. Results S. nigrum alkaloid could increase the ratio of adherence anadem between erythrocyte and tumor-cell and promote the erythrocyte membrane fluidity of the two tumor-bearing mice. Conclusion The inhibition of S. nigrum alkaloid on tumor can be accomplished through improving the erythrocyte membrane fluidity of two tumor-bearing mice and renewing erythrocyte immunity of tumor-bearing mice.

关 键 词：龙葵碱 红细胞免疫 膜流动性 荷瘤小鼠 

分 类 号：R286.91[医药卫生—中药学]