Schiff碱钴(Ⅱ)配合物催化PNPP水解研究  

Studies on PNPP hydrolysis catalyzed by Schiff base cobalt(Ⅱ) complexes

作　　者：王莹[1] 胡伟[1] 邹立科[2] 胡常伟[1] 

机构地区：[1]四川大学化学学院,成都610064 [2]四川理工学院化学系,自贡643000

出　　处：《四川大学学报（自然科学版）》2007年第1期145-149,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：四川省教育厅重点自然科学基金(2005D007)

摘　　要：在pH7．0～8．2和25℃下，用分光光度法研究了两种带冠醚环或吗啉环的Schiff碱钴（Ⅱ）配合物COL^1和COL^2，作为模拟水解金属酶，对羧酸酯水解的催化作用．通过对水解反应体系的特性吸收光谱的分析，提出了PNPP的催化水解的机理，并在此机理上建立了PNPP催化水解的动力学数学模型．讨论了两种Co（Ⅱ）配合物催化水解PNPP的性能，动力学和反应机理．Complexes of the Sehiff base with morpholino or aza-crown pendants, CoL^1 and CoL^2, as mimic hydrolytic metalloenzyme, were used in catalytic hydrolysis of earboxylie ester （PNPP） at pH7.0-8.2 and 25℃ . The mechanism of PNPP catalytic hydrolysis has been proposed based on the analytic result of specific absorption spectrum of reaction systems. A kinetic mathematical model, applied to the calculation of the kinetic parameter of PNPP catalytic hydrolysis, has been established based on the mechanism proposed. In this paper, the function, kinetics and mechanism of PNPP hydrolysis catalyzed by the two cobalt （ Ⅱ ） complexes have also been discussed.

关 键 词：Schiff碱钴(Ⅱ)配合物 模拟水解酶 动力学反应 羧酸酯 

分 类 号：O614.81[理学—无机化学]