吴茱萸超微饮片ITS序列指纹图谱的研究  

Study on ITS Sequence Fingerprint of Ultramicro Fructus Evodiae

作　　者：李顺祥[1] 蔡光先[1] 张平[2] 韦里俊[1] 黄丹[3] 

机构地区：[1]湖南省中医药研究院,长沙410006 [2]湖南师范大学 [3]湖南中医药大学,长沙410007

出　　处：《科技导报》2007年第5期42-46,共5页Science & Technology Review

基　　金：国家"十五"科技攻关项目(2001BA701A43);湖南省教育厅课题(04C452);湖南省卫生厅中医药科研基金课题(24303)

摘　　要：为探求吴茱萸超微饮片的品种鉴别方法,以来自不同产地的5个吴茱萸样品、7个疏毛吴茱萸和3个石虎样品制成的超微饮片为实验材料,用分子克隆方法获得并测定其ITS序列。标记它们的ITS1,5.8s,ITS2的全长序列,构建了吴茱萸的ITS序列指纹图谱,得出吴茱萸超微饮片3个不同植物来源种内相似度在98%以上;吴茱萸和其变种疏毛吴茱萸及石虎之间的ITS序列有显著的差异,可达27%,而疏毛吴茱萸和石虎的序列差异较小,但是在两组序列的对比中,发现有8个特异位点,显示了这两个亲缘关系比较相近的药材的区别。以ITS序列测定与分析可作为吴茱萸超微饮片的品种鉴定和质量控制的方法。To study the identification and evaluation methods of Ultramicro Fructus Evodiae, samples of Ultramicro Fructus Evodiae were taken, respectively, from fifteen kinds of Evodia Rutaecarpa （Juss.） Benth., and its varietas, their total DNAs were extracted arid cloned, and the ITS sequencee were determined by molecular biological techniques. The ITS1, 5.8S and ITS2 sequences of ultramicro Fructus evodiae from three plant species were obtained. The inner similitude of same species was above 98%, but the diversity among these sequences was about 27%. Analysis of ITS sequence fingerprint can provide an effective criterion for the identification of Ultramicro prepared herbs.

关 键 词：中药学 超微饮片 吴茱萸 ITS序列 品种鉴定 

分 类 号：R284[医药卫生—中药学]