油菜素内酯促进绿豆下胚轴不定根发生研究  

Stimulation of Brassinolide on Advititious Root Formation in Mung Bean

作　　者：刘灵[1] 李海航[1] 杨丽霞[1] 谢子华[1] 龙海涛[1] 

机构地区：[1]华南师范大学生命科学学院

出　　处：《科技导报》2007年第5期56-60,共5页Science & Technology Review

摘　　要：用10-2mol/L油菜素内酯浸泡绿豆种子,其幼苗下胚轴插条培养2d后,不定根长度、根数和生根范围均增加,分别比对照增加12.5%,27.4%和37.6%,插条基部细胞的吲哚丙酮酸脱羧酶、吲哚乙醛氧化酶和色氨酸转氨酶的比活性分别比对照提高120.6%,82.1%和54.7%,吲哚乙酸含量增加22.3%,插条基部细胞进入细胞周期S期的数目增加23%。结果表明,BR可能通过增加IAA含量,促进DNA合成和细胞分裂,从而促进不定根的发生。The pre-treatment of germinating seeds of mung bean with 10^-2 mol/L of brassinolide （BR） is found to help the advititious root formation and root growth in hypocotyl cuttings of mung bean. Root length, root numbers and rooting areas per cutting were increased by 12.5%, 27.4%, 37.6%, respectively, over the controls after two days of treatment. Enzyme activities of indole pyruvate decarboxylase, indole acetaldehyde oxidase and tryptophan aminotransferase were increased by 120.6%, 82.1% and 54.7%, respectively, and indole acetic acid （IAA） content was increased by 22.3% in the rooting zone of the cuttings. Cell numbers in S phase in the basal part of rooting zone was increased by 23% in the BR treatment. Results indicate that BR helps the rooting by increasing IAA contents

关 键 词：油菜素内酯 绿豆 不定根 吲哚乙酸 细胞周期 

分 类 号：Q945[生物学—植物学]