人工控制自然交尾条件下中国对虾父本的微卫星识别  

The identification of agnates of Fenneropenaeus chinensis naturally copulated with artificial control by using the microsatellite technology

作　　者：高焕[1] 孔杰[2] 于飞[3] 王伟继[2] 罗坤[3] 孟宪红[2] 刘萍[2] 张天时[2] 

机构地区：[1]中国科学院海洋研究所,青岛266071 [2]农业部海洋渔业资源可持续利用重点开放实验室中国水产科学研究院黄海水产研究所,青岛266071 [3]上海水产大学,200090

出　　处：《海洋水产研究》2007年第1期1-5,共5页Marine Fisheries Research

基　　金：国家863高技术研究发展计划项目(2005AA603210);国家自然科学基金(30500378)共同资助

摘　　要：人工控制自然交尾条件下,在保持中国对虾雌、雄5∶1的交配比率下,获得了两尾交尾并产生子代的雌虾,同时有4个疑似父本需要识别。利用3个微卫星引物及其组成的三重PCR技术对4个疑似父本进行了鉴别,准确的找到了相应的与雌虾交尾的雄虾。这为建立半同胞家系提供了一种新的技术和思路。Through keeping the female and male chinese shrimp （Fenneropenaeus chinensis） in the copulative ratio of 5 ： 1 under the condition of natural mating with artificial control, we obtained two copulating females of Chinese shrimp which had filial generation already. Simultaneously, there were four suspicious male parents needed to be identified. By using 3 microsatellite primers and the multiplex PCR technique, we identified exactly the corresponding male shrimp mated with the female shrimp among the four suspicious male parents. This study might provide a fresh train of thoughts and techniques for establishing the half-sib parentages.

关 键 词：中国对虾 亲子鉴定 微卫星 

分 类 号：Q111.22[生物学—普通生物学] Q959.223.63