淫羊藿苷对鸡胚成骨细胞增殖和分化的影响  

Effects of icariin on Proliferation and Differentiation of Osteoblasts from Chicken Embryo

作　　者：胡云峰[1] 侯加法[1] 邓益锋[1] 周振雷[1] 姚静[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095

出　　处：《中国农学通报》2007年第3期43-46,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"IGFs基因多态性分布与笼养蛋鸡骨质疏松症关系"(30270998);国家自然科学基金"蛋鸡髓质骨形成中雌雄激素对成骨细胞和破骨细胞作用及其分子机理"(30671546)

摘　　要：【研究目的】本文研究了淫羊藿苷对鸡胚成骨细胞增殖和分化的影响。【方法】将不同浓度的淫羊藿苷加入第二代成骨细胞中,分别于培养24、48、72h时,使用MTT法和PNPP法检测成骨细胞的增殖率和碱性磷酸酶(ALP)活性。【结果】淫羊藿苷促进鸡胚成骨细胞增殖和分化,但增殖作用不显著。其中当淫羊藿苷浓度为20ng/ml时,对成骨细胞增殖分化作用最强。【结论】淫羊藿苷能够显著促进鸡胚成骨细胞分化,对成骨细胞增殖有一定作用。[OBJECTIVE]This article is to study the effect of icariin on the proliferation and differentiation of osteoblasts of chicken embryo. [METHOD]The osteoblasts cultured with icariin of different concentration, MTT and PNPP methods were used to assay the proliferation and ALP activity of osteoblasts after 24, 48 h and 72 h. [RESULTS]The results showed that icariin stimulated the proliferation of osteoblasts and activity of ALP, especially at 20 ng/ml, but effects of icariin on proliferating rate of osteoblasts was not significant. [CONCLUSION]It revealed that effect of icariin on the differentiation of osteoblasts of chicken embryo was significant, and proliferation effect was limit.

关 键 词：淫羊藿苷 成骨细胞 增殖 碱性磷酸酶 

分 类 号：S853.74[农业科学—临床兽医学]