影响银白杨再生体系建立的因素分析  

Analysis on the Factors Affecting Regeneration System of Populus alba

作　　者：刘杰[1] 李慧[1] 陈晓阳[1] 

机构地区：[1]北京林业大学林木花卉遗传育种教育部重点实验室,北京100083

出　　处：《中国农学通报》2007年第3期156-159,共4页Chinese Agricultural Science Bulletin

基　　金：教育部重点科研项目"生长素诱导型GH3启动子/rolB融合基因及其在转基因毛白杨特异性表达机理研究"(01028)

摘　　要：为了获得银白杨离体再生体系的优化方案,通过对离体再生体系中银白杨无菌苗的获得、生芽培养基激素组合及诱导出芽、生根培养基激素组合及诱导生根进行了一系列优化试验。结果显示:影响银白杨植株再生的最主要因素是激素浓度和类型,其次是培养基种类和外植体类型;最利于银白杨再生植株的培养基条件为:芽增殖培养基以MS+6-BA1.0mg/L+NAA0.3mg/L+IBA0.6mg/L为宜,生根培养基以1/2MS+NAA0.05mg/L+IBA0.2mg/L为宜。A series of experiments are conducted that suitable hormone concentrations for explants to shoot and root on tissue culture medium were selected in order to find the best way to construct regenerative system of populus alba. The results show that the main factors affecting the efficiencies of regeneration system are hormone concentration and types as well as culture medium kinds and explants types. The best bud medium is MS＋6-BA1.0mg/L＋NAA0.3mg/L＋IBA0.6mg/L＋IBA0.4mg/L and the best rooting medium is 1/2MS＋NAA0.05mg/L＋IBA0.2mg/L

关 键 词：银白杨 再生体系 不定芽 

分 类 号：S813.3[农业科学—畜牧学]