哈密瓜内生拮抗细菌的筛选及其抑菌物质研究初报  

Studies on Screening Antagonistic Strains and Their Inhibitive Substance of Endophyte in Hami Melon Plants

作　　者：姜云琴[1] 罗明[1] 张祥林[2] 陈金焕[1] 

机构地区：[1]新疆农业大学农学院,乌鲁木齐830052 [2]新疆出入境检验检疫局,乌鲁木齐830063

出　　处：《中国农学通报》2007年第3期356-359,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然基金项目(30460005);新疆教育厅优秀青年学者科学研究奖励计划项目(XJEDU2004E06)

摘　　要：通过测定菌体及胞外分泌物抑菌活性,从378个哈密瓜内生细菌中筛选出了对瓜类细菌性叶斑病菌、疫霉病菌具有较强拮抗活性的菌株。对抗菌活性显著的197菌株的胞外抑菌活性物质进行了初步研究。研究显示:197菌株胞外分泌物的抗菌效价可达80倍;其抗菌活性物质为蛋白质类物质,可通过15%-90%饱和度的硫酸铵盐析,其中75%硫酸铵饱和度提取的粗蛋白的抗菌活性最强;该抗菌蛋白具有一定的耐热性,温度高于40℃后抑菌活性逐渐下降,100℃下仍能保持部分抑菌活性,120℃时全部丧失。Antagonistic strains from three hundred and seventy eight endophytic bacteria strains isolated in Hami melon plants that strongly showed the ability against Pseuodornonas syringae pv.melonis and Phytophthora drechsler were screened by examining inhibitive activity in vitro. The characteristic of extracellular secretive inhibitive substance produced by the #197 strain, one of the best antagonistic strains was studied. Results indicated that the extracellular secretion could strongly inhibit P. syringae and P. drechsler and its inhibitive activity still remained after the extracellular secretion was diluted to 80 times. It was confirmed that the antagonistic activities came from the protein secreted by the #197 strain. The filtrated #197 strain was precipitated in grades with the ammonium sulfate to draw the Anti-proteins, The crude proteins drawn with ammonium sulfate at 15%-90% and 75% saturation showed strong inhibitive activities. Antagonistic activities of the proteins decreased when the temperature was more than 40℃. When the temperature climbed to 100℃, some of the activities were kept, but at 120℃ it denaturalized completely.

关 键 词：哈密瓜 内生细菌 拮抗 筛选 抑菌物质 

分 类 号：S47[农业科学—农业昆虫与害虫防治]