子痫前期患者血清对氧磷酶基因多态性及其活力检测  

Detection of paraoxonase gene polymorphism and activity in preeclampsia

作　　者：罗美瑜[1] 李玉琴[1] 沈晓丽[2] 

机构地区：[1]福建医科大学福建省妇幼保健院,350001 [2]福建医科大学福建省立医院临床学院省心血管病重点实验室

出　　处：《中华围产医学杂志》2007年第1期19-23,共5页Chinese Journal of Perinatal Medicine

摘　　要：目的研究对氧磷酶1（paraoxonase1，PON1）基因R192Q位点多态性及PON1活力和血脂脂蛋白水平在子痫前期（preeclampsia，PE）发病中的相关性。方法共139例孕妇，其中PE组73例，包括轻度子痫前期（mild preeclampsia，MPE）34例，重度子痫前期（severe preeclampsia，SPE）39例，对照组为66例健康孕妇。应用全自动生化分析仪测定血脂脂蛋白，乙酸苯酯法测定PON1活力，聚合酶链反应（polymerase chain reaction，PCR）、限制性片段长度多态性（restriction fragment length polymorphism，RFLP）方法分析PON1 R192Q位点多态性。结果（1）SPE组胆固醇（cholesterol，CHOL）、甘油三脂（triglyeride，TG）、低密度脂蛋白（low density lipoprotein，LDL）和载脂蛋白B（apolipoproteinB，apoB）均比对照组高（P〈0．05），而高密度脂蛋白（high density lipoprotein，HDL）和载脂蛋白A（apolipoprotein A，apoA）比对照组低（P〈0．05）。MPE组TG、HDL、apoA和apoB与对照组差异显著（P〈0．05），CHOL和LDL与对照组差异无显著性（P〉0．05），与SPE组差异显著（P〈0．05）。（2）PE组PON1活力明显低于对照组（P〈0．05）。MPE组与SPE组比较差异无显著性（P〉0．05）。（3）PE组和对照组间的基因型变异频率和R、Q等位基因频率均有统计学差异（P均〈0．05）。（4）PON1活力比较，QQ基因型分别高于RR和RQ基因型（P均〈0．05），RQ基因型比RR基因型高（P〈0．05）。结论PE患者PON1活性下降。PON1基因R192Q位点多态性与PON1活力有关，QQ基因型PON1活力最高，其次是RQ和RR基因型。PON1 R192Q位点多态性与PE发病有关，R等位基因可能是其易感基因。Objective To address paraoxonase 1（PON1） gene polymorphism and its relationship with plasma paraoxonase activity, lipid and lipoprotein levels in preeclampsia（PE）. Methods One hundred and thirty nine gravidas were enrolled in this study including 73 women with PE among which 34 were mild preeclampsia（MPE） and 39 severe preeclampsia（SPE） and the rest 66 healthy gravidas as control. Lipid and lipoprotein were detected with automatic biochemistry analyzer. PON1 activity was measured spectrophotometrically with phenylacetate. Gene polymorphism of PON1 at site 192 was detected with polymerase chain reaction （PCR） and restriction fragment length polymorphism （RFLP）. Results （1）In the SPE group, the levels of cholesterol（CHOL）, triglyeride（TG）, low density lipoprotein （LDL） and apolipoproteinB （apoB） were higher than those of the healthy group （P〈0.05）, while that of the high density lipoprotein （LDH） and apolipoproteinA（apoA） was lower （P〈0. 05）. The levels of TG, HDL, apoB and apoA were significantly different between the mild preeclampsia （MPE） and healthy control （P〈0. 05）, those of CHOL and LDL were different between the MPE and SPE （P〈0. 05） and no significant differences was found between MPE and healthy control （P〉0.05）. （2） The PON1 activity was lower in PE group than in the control （P〈0. 05） and no difference between the SPE and MPE （P〉0. 05）. （3） The frequencies of genotype variation at 192 of PON1 gene and R, Q alleles were significantly different between the PE and control group （P〈 0. 05）. （4） The PON1 activity of PON1 QQ genotype was higher than that of RR and RQ genotype＇s（P〈 0.05） and that of RQ genotype＇s was higher than RR genotype＇s（P〈0. 05）. Conclusions The variant of 192 polymorphism site of PON1 gene is associated with the pathogenesis of PE, and R allele might be a susceptible gene to PE.

关 键 词：先兆子痫 血清 芳基二烷基磷酸酶 基因多态现象 

分 类 号：R714[医药卫生—妇产科学]