高效液相色谱法测定五味风湿胶囊中栀子苷的含量  

HPLC Determination of Gardenoside in Wuwei Fengshi Capsules

作　　者：王秀兰[1] 邰文泉[1] 吴龙堂[1] 

机构地区：[1]内蒙古民族大学蒙医药学院,内蒙古通辽028041

出　　处：《中国现代应用药学》2007年第1期59-60,共2页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立五味风湿胶囊中栀子苷的含量测定方法。方法采用HPLC法，VP-ODS柱，流动相为乙腈-0．1％磷酸（15：85），检测波长为238nm。结果栀子苷线性范围在0．15—0．64μg，r=0．9996，平均回收率98．32％，RSD=2．1％。结论此方法可用于五味风湿胶囊质量控制。OBJECTIVE To establish a method to determine the content of gardenoside in Wuwei Fengshi Capsules. METHODS HPLC conditions were as follows： VP-ODS column, a mobile phase of CH3CN-0.1% H3PO4 （15： 85） ,and the wavelength at 238 nm. RESULTS Gardenoside was linear within the range of 0.15 - 0.64μg with a correlation coefficient of 0.9996. The average recovery percentage was 98.32% and RSD was 2.19%. CONCLUSION The method can be used effectively for the quality control of Wuweifengshi Capsules.

关 键 词：五味风湿胶囊 栀子苷 高效液相色谱 

分 类 号：R917.792.1[医药卫生—药物分析学]