细胞外基质对大鼠MSCs生物学活性的影响  

Biological effects of the extracellular matrix on rat bone marrow mesenchymal stem cells

作　　者：徐蕴[1] 肖琼[1] 田铧[1] 张立平[1] 田广平[1] 

机构地区：[1]山东大学医学院解剖与组织胚胎研究所,山东济南250012

出　　处：《中国现代普通外科进展》2007年第1期26-28,36,共4页Chinese Journal of Current Advances in General Surgery

基　　金：山东省自然科学基金资助项目(Y2005C14)

摘　　要：目的:观察细胞外基质多聚赖氨酸对大鼠骨髓间充质干细胞(MSCs)生长特性的影响,以寻求体外培养扩增MSCs更理想的方法。方法:用贴壁法分离获得大鼠MSCs,分别接种于涂布和未涂布多聚赖氨酸的培养器皿上,显微镜下观察细胞贴壁速度、生长和形态特点,流式细胞仪分析细胞生长周期。结果:MSCs在用多聚赖氨酸处理过的培养器皿上贴壁、增殖都较普通组快,细胞形态好,细胞活力和长势较普通组活跃。结论:细胞外基质多聚赖氨酸能明显促进MSCs的贴壁和增殖,有助于MSCs的体外扩增培养。Objective： To investigate the biological effects of polylysine on bone marrowmesenchymal stem cells. Methods： MSCs were isolated from Wistar rats bone marrow by adherence methods, and were cultured in flasks coated with polylysine or not respectively. Then we observed the cell adherence and growth characters by microscopy and flow cytometry, Results： Compared with the control group, the MSCs growing on the polylysine showed more rapid adhesion and proliferation, as well as more typical morphous. Concluusion： The polylysine as an extracellular matrix can significantly increase the adhesion and proliferation capacity of MSCs in vitro.

关 键 词：骨髓间充质干细胞 多聚赖氨酸 细胞培养 

分 类 号：Q2[生物学—细胞生物学]