亚硝化作用菌种的分离筛选及条件选择  

Study on Isolation Screening and Condition Selection of Nitrobacteria

作　　者：廖雪义[1] 马光庭[2] 蓝荣 李献[2] 

机构地区：[1]襄樊学院化学与生物科学系,湖北襄樊441053 [2]广西大学农学院,广西南宁530005 [3]广东省中山市杨氏南北鲜果有限公司,广东中山528451

出　　处：《安徽农业科学》2007年第5期1259-1261,共3页Journal of Anhui Agricultural Sciences

摘　　要：通过富集培养、硅胶平板分离法和亚硝化作用试验,筛选出1株亚硝化速率较高的菌株(编号为N4,下同)。经初步鉴定其为亚硝化单胞菌(Nitrosospira sp.),并对它作了亚硝化作用条件试验。试验结果表明:其最佳的亚硝化作用条件为温度30℃、pH值7.5～8.0,初始氨氮浓度100～150 mg/L,通气量为摇床转速110 r/min,碱度为NaHCO3浓度1 700 mg/L。在此条件下,接种浓度为15%,培养24 h,氨氮去除率为99.47%,亚硝酸盐氮积累量可达到116.65 mg/L。A nitrobacteria of high nitrosification rate was isolated in this paper. After preliminary examination, it was concluded that the bacteria was Nitrosospira sp. Results showed that the optimal nitrosification condition was 25-30℃, pH 7.5-8.0, 110 r/min, 1 700 mg/L NaHC03 alkalinity, 150 mg/L NH4^＋- N. Under the above condition and 15 % inoculation rate, after 24 h, the removal efficiency of NH4^＋- N reached 99.47 %, and accumulative volume of NO2^-- N was 116.65 mg/L.

关 键 词：亚硝化作用 亚硝化细菌 菌种分离筛选 条件选择 

分 类 号：Q93-337[生物学—微生物学]