禽霍乱蜂胶灭活疫苗研制中的铁螯合剂适宜浓度确定  

Development of a Novel Broad-spectrum Propolis Adjuvant Inactivated Vaccine against Fowl Cholera

作　　者：徐海军[1] 刘俊良[1] 胡方宏 陈乃富[1] 孙协军[1] 

机构地区：[1]皖西学院化学与生命科学系 [2]安徽省皖西白鹅疫病监测中心,安徽六安237000

出　　处：《安徽农业科学》2007年第5期1378-1379,共2页Journal of Anhui Agricultural Sciences

基　　金：安徽省教育厅自然科学研究项目"新型广谱禽霍乱蜂胶灭活疫苗的研制(2006KJ416B)"

摘　　要：以含4%鸡血清的营养琼脂为培养基,进行了铁螯合剂2,2’-联吡啶对禽多杀性巴氏杆菌C48-3株的抑菌试验。结果表明,对于含4%鸡血清的营养琼脂培养基,铁螯合剂2,2’-联吡啶的合适添加量为200μmol/L。Nutritious agar supplemented with 4% chicken serum was used as culture media. Suppressive effect of iron-chelator 2,2＇-dipyridyl on pasteurella multocida strain C48 -3 was studied in order to find an easy method to confirm the appropriate concentration of iron -chelator supplemented in a specific culture media to induce expression of cross-protective factor （CPF） by pasteurella multocida. The result indicated that with this method, an appropriate concentration of iron-chelator could be found out for specific culture media to induce expression of CPF. As for nutritious agar culture media supplemented with 4% chicken serum, the appropriate concentration of 2,2＇-dipyridyl was 200 μmol/L.

关 键 词：铁 螯合剂 2 2’-联吡啶 多杀性巴氏杆菌 

分 类 号：S851.347.2[农业科学—预防兽医学]