组成型表达的ClpB基因提高番茄植株的耐冷性  

Improvement of Chilling Tolerance in Tomato(Lycopersicon esculentum Mill.) Plant by Constitutive Expression of ClpB Gene

作　　者：秦佳[1] 杨金莹[2] 伊淑莹[1] 赵春梅[1] 李明辉[1] 刘箭[1] 

机构地区：[1]山东师范大学生命科学学院,济南250014 [2]中国石油大学(华东)生物工程与技术中心,山东青岛266555

出　　处：《植物生理学通讯》2007年第1期16-20,共5页Plant Physiology Communications

基　　金：国家自然科学基金(30270132);山东省青年科学基金。

摘　　要：用农杆菌介导法将CaMV35S启动子驱动的ClpB cDNA导入番茄,并比较了转基因和未转基因番茄的抗冷能力。当受冷胁迫后,转基因番茄比未转基因番茄表现出较轻的冷胁迫症状,并维持较高的PSII水平。ClpB cDNA under the control of CaMV35S promoter was introduced into tomato （Lycopersicon esculentum） plants by Agrobacterium tumefactions-mediated. The chilling tolerances of transgenic and nontransformed tomato lines were evaluated. After exposure to chilling stress, transgenic plants exhibited lighter symptoms of chilling injury and kept higher values of Fv/Fm in PSII than those of non-transformed plants. All results indicated that the chilling tolerance in transgenic tomato plants was improved.

关 键 词：CLPB 耐冷性 转基因番茄 PSⅡ 

分 类 号：S641.2[农业科学—蔬菜学]