柴胡总皂甙对戊四氮慢性点燃大鼠海马γ-氨基丁酸表达的影响  

Effect of saikosaponin on GABA of hippocampus of chronic kindling induced by pentylenetetrazol in rats

作　　者：谢炜[1] 鲍勇[2] 于礼建[2] 

机构地区：[1]南方医科大学南方医院中医内科,广东广州510515 [2]南方医科大学中医药学院,广东广州510515

出　　处：《西安交通大学学报（医学版）》2007年第1期21-24,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：广东省中医药局科研课题(No.401001;1040091)

摘　　要：目的研究柴胡总皂甙对戊四氮(PTZ)慢性点燃癫痫模型大鼠海马区γ-氨基丁酸(GABA)表达的影响。方法48只健康SD大鼠被随机均分为6组,即空白对照组(A组)、生理盐水组(B组)、丙戊酸钠(VPA)组(C组)和柴胡总皂甙高、中、低三种剂量组(D组、E组、F组)。除A组不做处理外,其他各组采用腹腔注射PTZ慢性点燃造模,造模同时给予VPA、柴胡总皂甙等不同处理因素,连续4周后取脑组织切片进行GABA免疫组化染色,从阳性细胞数、灰度值分析结果。结果B组海马CA1、CA2、DG区的阳性细胞数高于其他各组(P<0.05),而各区阳性细胞灰度值低于其他各组(P<0.05)。结论柴胡总皂甙可以影响PTZ点燃大鼠海马的GABA水平,发挥抑制PTZ对大鼠点燃的作用。Objective To study the effect of active ingredients （saikosaponin） of bupleurum chinensis D C. on GABA of hippocampus of chronic kindling induced by pentylenetetrazol （PTZ） in rats. Methods Forty-eight healthy Sprague-Dawley rats were randomly divided into six groups with eight in each group： normal control （Group A）, normal saline （NS） group （Group B）, sodium valproate （VPA） group （Group C）, group of large-dose saikosaponin （Group D）, group of medium-dose saikosaponin （Group E）, and group of small-dose saikosaponin （Group F）. Except Group A to be controlled, the other groups were given different treatments when chronically kindled by PTZ. The process of the test lasted for 4 weeks. After 4 weeks＇ administration the brain tissues were sampled, sliced and stained by enzyme immunohistochemistry（EIH）. The results were analyzed according to the positive cell population and gray value. Results The positive cell population of CA1, CA2 and DG in group B was significantly different from that in the other groups（P〈0.05）. The gray value of CA, CA2 and DG in group B was significantly different from that in the other groups （P〈0.05）. Conclusion Saikosaponin can influence GABA of hippocampus of rats ,and inhibit chronic kindling effect induced by PTZ.

关 键 词：柴胡总皂甙 戊四氮 癫痫 Γ-氨基丁酸 大鼠 

分 类 号：R285.5[医药卫生—中药学]