TGF-β_1中和抗体在预防肌腱黏连中的应用  

The preventive effect of TGF-β_1 neutralizing antibody on flexor tendon adhesion from operation

作　　者：王金堂[1] 李军[1] 贾光辉[1] 张小卫[1] 张银刚[1] 

机构地区：[1]西安交通大学医学院第一附属医院骨科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2007年第1期83-85,102,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省自然科学基金资助项目(No.y100323004)

摘　　要：目的探讨应用TGF-β1多克隆抗体抑制术后屈指肌腱黏连的效果。方法将108只来亨鸡随机分成3组,行中趾屈指肌腱切断吻合术,术中分别滴加生理盐水或不同浓度的TGF-β1多克隆抗体,于术后1、3、8周取肌腱行HE染色、Masson胶原染色、苦味酸-天狼星红染色和TGF-β1免疫组化染色。结果TGF-β1多克隆抗体10μg/mL组炎症过程缩短,塑形过程加速;TGF-β1多克隆抗体5μg/mL组、10μg/mL组Ⅰ胶原含量减少,且Ⅰ/Ⅲ胶原比例下降,TGF-β1的表达降低。结论TGF-β1多克隆抗体可以有效地抑制TGF-β1在肌腱损伤修复过程中的作用,减少肌腱的黏连、瘢痕组织的形成,这可能是治疗屈指肌腱损伤术后黏连潜在的有效方法之一。Objective To investigate the preventive effect of TGF-β1 neutralizing antibody on flexor tendon adhesion from operation. Methods Totally 108 leghon cocks performed anastomosis operation were divided into three groups randomly： normal saline （control group）, 5μg/mL group, and 10μg/mL group of TGF-β1 antibody. At week 1, 3, and 8, respectively, after operation, HE staining, Masson staining, Sirius red-polarization staining and TGF-β1 immunohistochemistry staining were used. Results Compared with the control group, the 10μg/mL group had less shortened progress of inflammation and accelerated progress of molding; in the experimental groups （5μg/mL group, 10 μg/mL）, the density of the collagen Ⅰ type was smaller, the ratio of Ⅰ/Ⅲ collagen and expression of TGF-β1 decreased. Conclusion TGF-β] multiclonal neutralizing antibody can effectively inhibit the function of TGF-β1 during the flexor tendon repair, reduce tendon adhesion and scar formation, suggesting that it is a latent and effective method for preventing flexor tendon from adhering after operation.

关 键 词：TGF-Β1抗体 肌腱 黏连 显微外科手术 

分 类 号：R68[医药卫生—骨科学]