PAI-1在糖尿病大鼠肾脏表达的动态观察  

A Dynamic Study of Plasminogen Activator Inhibitor-1 Expression in Kidney of Streptozocin Induced Diabetic Nephropathy Rats

作　　者：孙迪[1] 张晓林[1] 谢汝佳[1] 杨婷[1] 杨勤[1] 

机构地区：[1]贵阳医学院病理生理学教研室,贵州贵阳550004

出　　处：《贵阳医学院学报》2007年第1期15-18,共4页Journal of Guiyang Medical College

基　　金：教育部春晖国际合作项目(Z2005-52018);贵州省高层次人才基金资助(2006)

摘　　要：目的:动态观察大鼠糖尿病肾病发生、发展过程中纤溶酶原激活物抑制物-1(plasminogen activatorinhibitor-1,PAI-1)在肾脏的表达变化,旨在探讨PAI-1在糖尿病肾病(diabetic nephropathy,DN)肾纤维化发病机制中的作用。方法:链脲菌素(streptozocin,STZ)诱导大鼠糖尿病肾病,以免疫组织化学及荧光实时定量PCR(RT-PCR)的方法动态观察糖尿病肾病发生、发展过程中大鼠肾脏PAI-1蛋白及mRNA的表达。结果:PAI-1蛋白表达从糖尿病2周起较正常对照组明显增多(P<0.05),且随着糖尿病进展有逐渐增加的趋势,阳性表达主要位于肾小管上皮细胞的胞浆内。用RT-PCR的方法检测大鼠肾脏PAI-1 mRNA的表达,结果显示16周DN组大鼠肾组织中PAI-1 mRNA的表达显著高于正常对照组大鼠(P<0.05)。结论:PAI-1表达上调可能参与了糖尿病肾病中肾脏纤维化形成的过程。Objective： To study the role of plasminogen activator inhibitor-1 （PAI-1） in development of renal fibrosis in diabetic nephropathy. Methods： Rat diabetic nephropathy was induced by using streptozocin （STZ） tail intravenous injection. The expression of PAI-1 protein and mRNA were examined with immunohistochemical technique and RT-PCR. Results： With the progression of diabetic nephropathy renal fibrosis, the expression of PAI-1 protein and mRNA in kidney of diabetic rats elevated, and in the 16^th week of the experiment, they were significantly higher than those in rats of control group（P 〈0. 05）. Conclusions： In STZ-induced diabetic rats, the up-regulated PAI-1 expression appears to take part in the process of the renal fibrosis.

关 键 词：糖尿病肾病 纤溶酶原激活物抑制物1 链脲菌素 大鼠 Wistar 

分 类 号：R587.1[医药卫生—内分泌]