次氯酸钠水浴处理对板栗呼吸和几种酶活性的影响  被引量:14

Effects of Sodium Hypochlorite Solution Water-Curing Treatment on Respiration and Amylase Activity of Chestnut

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作  者:杨小胡[1] 梁丽松[1] 王贵禧[1] 石雪晖[2] 

机构地区:[1]中国林业科学研究院林业研究所国家林业局林木培育重点实验室 [2]湖南农业大学园艺园林学院,长沙410128

出  处:《林业科学》2007年第2期37-42,共6页Scientia Silvae Sinicae

基  金:国家林业局"948"项目(2004-4-54)

摘  要:以‘燕昌’板栗为试材,研究采后不同浓度NaClO水浴处理对板栗贮藏生理代谢的影响。结果表明:NaClO水浴处理使板栗呼吸强度和淀粉酶活性降低,乙醇脱氢酶(ADH)活性升高;NaClO水浴处理使板栗内部PPO活性降低,对外部PPO活性的影响与NaClO浓度相关,1000mg.L-1NaClO处理使板栗外部PPO活性一直高于对照,而100mg.L-1NaClO和500mg.L-1NaClO处理使外部PPO活性降低;NaClO水浴处理使板栗内部POD活性降低,对外部POD活性的影响与NaClO浓度相关,1000mg.L-1NaClO处理使板栗外部POD活性增加,其他处理在贮藏前期降低了外部POD活性,但在后期使外部POD活性升高;100mg.L-1NaClO和500mg.L-1NaClO处理使板栗SOD活性升高,而1000mg.L-1NaClO处理则使SOD活性降低;NaClO处理板栗的CAT活性变化趋势与对照相似,但提高了CAT活性。In this paper, the effects of different concentrations ( 100, 500, 1 000 mg·L^-1 ) of sodium hypochlorite solution water-curing treatment on physiological changes of chestnut ( Castanea moUissima cv. Yanchang) were studied during postharvest MA storage. The results were as follows: 1 ) NaClO water-curing treatment could decrease respiratory intensity and amylase activity, but increase ADH activity of chestnut; 2) NaClO water-curing treatment could inhibit intra-PPO activity, extra-PPO activity was related with concentrations of NaClO solution. The extra-PPO activity of chestnut treated with 1000 mg·L^-1 NaClO solution was higher than that of CK at all times. However, 100 mg·L^-1 or 500 mg·L^-1 NaClO solution water-curing treatment made extra-PPO activity lower than CK. 3) NaClO water-curing treatment could restrain intra-POD activity, extra-POD activity was related with concentrations of NaClO solution. Extra-POD activity of chestnut treated with 1 000 mg·L^-1 NaClO solution was higher than that of CK. However, extra-POD activity was lower in the early storage and higher in the later treated with 100 mg·L^-1 or 500 mg·L^-1 NaClO solution. 4) SOD activities were higher than CK by 100 mg·L^-1 NaClO or 500 mg·L^-1 NaClO solution treatment, but SOD activity was lower than CK by 1 000 mg·L^-1 NaClO solution treatment. 5 ) There were no differences between NaClO water-curing treatments and CK on the changing trends of CAT activities, but CAT activity was improved by NaClO water-curing treatment.

关 键 词:板栗 次氯酸钠 水浴处理 呼吸强度 酶活性 

分 类 号:S718.43[农业科学—林学]

 

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