天花粉蛋白抑制HeLa细胞增殖的实验研究  

Experimental Study of Trichosanthin's Effect on Hela Cells Proliferation

作　　者：黄益玲[1] 黄利鸣[1] 惠燕[1] 鲁华[1] 

机构地区：[1]三峡大学医学院,湖北宜昌443002

出　　处：《时珍国医国药》2007年第2期280-281,共2页Lishizhen Medicine and Materia Medica Research

基　　金：湖北省教育厅重大科研项目(No.Z200513002);湖北省教育厅重大科研项目(No.2002Z53004);湖北省科技攻关项目(No.2005AA401C57)

摘　　要：目的研究天花粉蛋白(TCS)对人宫颈癌HeLa细胞生长的抑制作用,探讨TCS抗肿瘤的作用机制。方法采用MTT法检测TCS对Hela细胞的抑制作用,形态学观察、DNA电泳及流式细胞仪检测TCS对HeLa细胞的诱导凋亡作用。结果TCS对HeLa细胞的生长具有明显的抑制作用,显微镜下可见细胞圆缩,胞浆凝聚等凋亡细胞的形态学改变,DNA凝胶电泳呈梯级格局,流式检测出现凋亡峰,凋亡比例具有剂量和时间依赖性。结论天花粉蛋白通过诱导细胞发生凋亡而抑制宫颈癌HeLa细胞的增殖。Objective To study the inhibitory effect of trichosanthin（Tcs） on Hela cells and its anti -tumor mechanism. Methods The antiproliferative effect of TCS on Hela cells was measured with MTT assay. Light microscopy was used to observe the morphology changes , DNA agarose electrophoresis and FCM analysis were carried out to examine the induced apoptosis. Results The proliferation of HeLa cells was significantly inhibited by TCS, Hela cells showed a serial of characteristic changes of apoptosis such as cell volume shrinkage , chromatin condensation; the occurrence of DNA ladder and apoptosis late of Hela cells was dose and time dependent. Conclusion Trichosanthin can inhibit the proliferation of Hela cells by its induction of apoptosis.

关 键 词：天花粉蛋白 HELA细胞 细胞凋亡 

分 类 号：R962[医药卫生—药理学]