壮药战骨高效液相色谱指纹图谱的研究  

Study on Fingerprint Chromatograms of Zhangu by High Performance Liquid Chromatography

作　　者：李小燕[1] 杨立芳[1] 邓光辉[1] 罗伟强[1] 潘洪平[2] 韦冬萍[1] 

机构地区：[1]广西民族大学化学与生态工程学院,广西南宁530006 [2]广西壮族自治区人民医院,广西南宁530021

出　　处：《时珍国医国药》2007年第2期287-290,共4页Lishizhen Medicine and Materia Medica Research

基　　金：广西壮族自治区教育厅科学基金资助项目(No.[2004]20号)

摘　　要：目的采用高效液相色谱法对壮药战骨药材进行指纹图谱的研究,为战骨药材质量标准的提升提供实验依据。方法实验采用Phenomenex Gemini C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.05 mol/L醋酸水溶液为流动相进行二元梯度洗脱,流速1.0 ml/min,柱温25℃,检测波长274 nm。结果以23个共有峰为评价指标,精密度和重复性实验中各共有峰相对保留时间和相对峰面积的RSD均小于3%,符合有关规定要求。结论此方法操作简单,精密度、稳定性和重复性良好,有助于战骨药材的质量控制。Objective The fingerprint chromatograms were established for quality evaluation of traditional Zhuang nationality medicine -Zhangu （ the root of Premna fulva craib） by high performance liquid chromatography. Methods The analysis was performed on a Phenomenex Gemini C18 column（4.6 mm ×250 mm,5μm） with acetonitrile-0.05 mol/L ethanoic acid as mobile phase in a gradient mode at a flow rate of 1.0 mL/min, and at a column temperature of 25℃. The detection wavelength was 274nm. Results Twenty three peaks were selected as the common fingerprint peaks. The relative standard deviations for relative retention values and relative peak areas were less than 3% in the precision and repeated test. Conclusion The method is reliable and can be helpful in the quality control of Zhangu.

关 键 词：战骨 黄毛豆腐柴 指纹图谱 高效液相色谱法 壮药 

分 类 号：R282.5[医药卫生—中药学]