圣和散对脑胶质瘤细胞诱导分化的研究  

Inducing Differentiation Effect of Shenghe Powder on C6 Brain Glioma Cells

作　　者：夏跃胜[1] 王建华[1] 吴永忠[2] 王欢[3] 

机构地区：[1]中国人民解放军总后勤部第五职工医院,山西闻喜043801 [2]中国人民解放军第四军医大学唐都医院,陕西西安710038 [3]山西中医学院,山西太原030024

出　　处：《时珍国医国药》2007年第2期290-291,共2页Lishizhen Medicine and Materia Medica Research

基　　金：山西省科技发展计划项目(No.002059)

摘　　要：目的观察圣和散对脑胶质瘤C6细胞株体外生长及诱导分化的作用。方法用圣和散处理脑胶质瘤C6细胞,采用甲基噻唑基四唑(MTT)比色法检测圣和散对C6细胞增殖的抑制作用,以免疫组化SABC法检测胶质纤维酸性蛋白(GFAP)和c-myc表达。结果圣和散可显著抑制C6细胞体外增殖,并呈现时间、剂量依赖性。在终浓度为5 mg/ml和10 mg/ml时,对C6增殖的抑制率达(49.62±1.13)%和(69.38±1.42)%,明显优于对照组(P<0.01)。免疫组化可见SHP组较对照组GFAP表达明显增强,c-myc表达下降。结论圣和散对胶质瘤C6细胞系的增殖有明显的抑制作用,能通过诱导分化降低脑胶质瘤细胞恶性程度。Objective To investigate the effects of Shenghe Powder（SHP） on the proliferation and inducing differentiation of brain glioma C6 cell line in vitro. Methods Brain glioma C6 cell line was co - cultured with SHP. Methyl thiagoly tetragolium （MTT） was used to measure the level of the proliferation of C6 cultured with SHP. The expressions of glial fibrillary acidic protein （GFAP） and c - myc of C6 cells were detected by immunohistochemical SABC method. Results The proliferation of C6 was obviously inhibited by SHP in a concentration - and time - dependent manner. The inhibitory rate was （49.62 ± 1. 13 ） % and （69.38 ± 1.42） % in SHP at 5mg/ml and 10 mg/ml,compared with the control group, there was a significant difference（ P 〈 0. 01 ） . The expression of GFAP was obviously enhanced and c - myc decreased significantly in C6 cells cultured with SHP. Conclusion SHP can inhibit the proliferation of C6 and decrease malignant degree by inducing differentiation of the C6 cells .

关 键 词：圣和散 脑胶质瘤 分化 

分 类 号：R285.5[医药卫生—中药学]