11种中药对小鼠淋巴细胞活性及腹腔巨噬细胞功能的影响  

Effect of 11 Traditional Chinese Herbs on Proliferation of Splenic Lymphocyte and Function Phagocytosis of Peritoneal Macrophage

作　　者：绳娟[1] 洪铁[1] 

机构地区：[1]吉林大学药学院,吉林长春130021

出　　处：《时珍国医国药》2007年第2期390-392,共3页Lishizhen Medicine and Materia Medica Research

摘　　要：目的研究具有免疫调节作用的11种中药水提取物,对小鼠脾淋巴细胞增殖活性以及腹腔巨噬细胞吞噬功能的影响。方法采用体外检测不同浓度的11种中药水提取物对正常、ConA及LPS诱导的小鼠脾淋巴细胞增殖作用的影响,运用中性红法检测巨噬细胞的吞噬功能。结果黄芩水提取物对正常淋巴细胞的增殖活性无明显影响,对ConA及LPS诱导的脾淋巴细胞增殖活性有显著的抑制作用,其IC50分别为16.54,19.00μg/ml;蒺藜及罗布麻水提取物能够显著促进巨噬细胞吞噬功能。结论黄芩水提取物对小鼠脾淋巴细胞增殖活性具有一定的调节作用,蒺藜及罗布麻对巨噬功能有一定的调节作用。Objective To investigate the immune modulatory effect of water extract of 11 traditional Chinese herbs on the proliferation of splenic lymphocyte and function phagocytosis of peritoneal macrophage. Methods The pattern of concanavalin A （ConA） or lipopolysaccharide（1.3%） -induced proliferative response of murine lymphocytes was assessed. Cell proliferation was measured by the XTT （2,3-bis[2 -4-nitro-5-suffophenyl] -2H -tetrazolium-5-carboxanilide assay in the normal cells and the cells treated with ConA or L PS, then measured the proliferation after treated with water extract of 11 traditional Chinese herbs（ The final concentration of water extract of 11 traditional Chinese herbs ： 100.0 ,50.0,25.0,12.5 μg/ml）. Peritoneal macrophage stained red with Neutral Red Dye. The function phagocytosis of peritoneal macrophage after treated with water extract of 11 traditional Chinese herbs for 12 hours was measured by the dose of Neutral Red Dye that they ingested. Results The results showed that there was no effect of water extract of 11 traditional Chinese herbs on normal lymphocytes. The water extract of Scutellaria baicalensis had noticeable inhibitory effect on the lymphocytes treated with ConA or LPS. The inhibition of the proliferation of lymphocytes treated with ConA after exposure to Scutellaria baicalensis was dose -dependent, its 50% inhibitory concentration（ IC50 ） was 16.54 μg/ ml. The corr-inhibition ratio was 41%, 63%, 70%, 78%. The inhibition of the proliferation of lymphocytes treated with LPS after exposure to Scutellaria baicalensis was dose-dependent, too. Its IC50 was 19.00 μg/ml. The corr-inhibition ratios were 42%, 59%, 64%, 80%. The water extract of Tribulus terrestris and Bluish dogbane enhanced peritoneal macrophage to ingeste Neutral Red Dye, it meant that the water extract of the two traditional Chinese herbs reinforced the function phagocytosis of peritoneal macrophage. Conclusion The water extract of Scutellaria baicalensis possesses the modulatory effect on murinelymphol

关 键 词：黄芩 蒺藜 罗布麻 淋巴细胞 巨噬细胞 

分 类 号：R285.5[医药卫生—中药学]