TaqMan低密度表达芯片检测DNA损伤修复基因在原发胶质瘤中的表达研究  

Expression analyses of multiple DNA repair genes in glioma by TaqMan low-density array

作　　者：姜政[1] 胡锦[3] 李新钢[2] 卢大儒[4] 江玉泉[2] 周伟[5] 

机构地区：[1]山东大学齐鲁医院神经外科实验室,济南250012 [2]山东大学齐鲁医院神经外科,济南250012 [3]上海交通大学第六人民医院神经外科 [4]复旦大学生命科学院遗传学研究所遗传工程国家重点实验室 [5]山东省肿瘤防治研究院放疗科

出　　处：《中华神经外科杂志》2007年第2期91-95,共5页Chinese Journal of Neurosurgery

基　　金：国家自然科学基金资助项目（30371459）,山东省卫生系统杰出学科带头人资助项目

摘　　要：目的 运用低密度表达谱芯片检测人脑原发胶质瘤组织中DNA损伤修复基因的表达情况，进一步分析其表达变化的意义。方法 使用TaqMan低密度表达芯片技术检测27个DNA损伤修复基因在40例不同级别原发胶质瘤组织和10例正常脑组织中的表达情况，并通过统计学分析其在不同级别胶质瘤和正常脑组织中的表达差异。结果 与正常脑组织相比较，有13个DNA损伤修复基因在Ⅱ、Ⅲ、Ⅳ级胶质瘤中均表达下调，包括ERCCl、ERCC2、ERCC3、ERCCA、MGMT、MLHl、MLH3、NTHLl、OGGl、RADS0、SMUGl、XRCCA、XRCC5（P〈0．05）。MSH2、MSH6、NUDTl和XRCC3只在Ⅱ级和Ⅲ级胶质瘤中表达下调；MREllA和MUSSl只在Ⅲ级和Ⅳ级胶质瘤中表达下调。PMS2、RAD52和XRCC1只在Ⅲ级胶质瘤中表达下调，而UNG只在Ⅱ级中表达下调。结论 TaqMan低密度表达芯片为多个基因的同时定量表达提供了一种准确、快速、有效的多变量检测技术，可用于发现新的肿瘤相关基因。大量的DNA损伤修复基因的表达下调，与胶质瘤的发生密切相关。Objectives To detect the expression of DNA repair genes in primary glioma tissues by low-density array, and to analyze the significance of the expression. Methods We described the novel TaqMan low-density array to examine the expression of 27 genes in the DNA repair systems in different grade glioma tissues from 40 patients and the compatible normal brain tissues from 10 non-glioma patients. The difference of expression in glioma tissues compared to the normal brains was analyzed by the statistic method. Results The expression of 13 genes were all significantly down-regulated in different grade gliomas （ Ⅱ ,Ⅲ, Ⅳ） compared to normal brain tissues including ERCC1, ERCC2, ERCC3, ERCCA, MGMT, MLH1, MLH3, NTHL1, OGG1, RAD50, SMUG1, XRCCA, XRCC5 （P 〈 0.05）. MSH2, MSH6, NUDT1 and XRCC3 were only significantly lower in grade （ Ⅱ , Ⅲ ） glima, and MREllA and MUSS1 were only significantly lower in grade （Ⅲ, Ⅳ ） glioma, compared to normal brain tissues. PMS2, RAD52 and XRCC1, were only significantly lower in grade Ⅲ glioma, and UNG was only significantly lower in grade Ⅱ glioma. Conclusion TaqMan low-density array provides an effective multivariate technique for examining the expression of DNA repair genes in RNA isolated from glioma tissues and can identify tumor specific genes. The down-regulation of multiple DNA repair genes may be associated with the initiation and development of glioma.

关 键 词：神经胶质瘤 DNA修复 实时定量PCR 

分 类 号：R686[医药卫生—骨科学]